Detailed information of TA system TA028699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2189873..2190501
• Replicon: chromosome
• Accession: NC_019973
• Organism: Mesorhizobium australicum WSM2073

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0KGV9
• Locus tag: MESAU_RS10485
• Protein ID: WP_015316022.1
• Coordinates: 2190115..2190501 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MESAU_RS10480
• Protein ID: WP_041163682.1
• Coordinates: 2189873..2190118 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESAU_RS10455	2185778..2186866	-	1089	WP_015316016.1	AbrB family transcriptional regulator	-
MESAU_RS10460	2186946..2187254	-	309	WP_015316017.1	antibiotic biosynthesis monooxygenase	-
MESAU_RS10465	2187313..2188098	-	786	WP_015316018.1	SDR family oxidoreductase	-
MESAU_RS10470	2188170..2188607	-	438	WP_015316019.1	DsrE family protein	-
MESAU_RS10475	2188709..2189806	+	1098	WP_015316020.1	hypothetical protein	-
MESAU_RS10480	2189873..2190118	+	246	WP_041163682.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MESAU_RS10485	2190115..2190501	+	387	WP_015316022.1	type II toxin-antitoxin system VapC family toxin	Toxin
MESAU_RS10490	2190508..2191434	-	927	WP_041163683.1	cation diffusion facilitator family transporter	-
MESAU_RS10495	2191461..2193650	-	2190	WP_015316024.1	anthranilate synthase	-
MESAU_RS10500	2193872..2194927	-	1056	WP_015316025.1	succinylglutamate desuccinylase/aspartoacylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14222.34 Da
• Isoelectric Point: 6.7284

>T28699 WP_015316022.1 NC_019973:2190115-2190501 [Mesorhizobium australicum WSM2073]
VIYLLDTNAVIAVIKGDASLLALLKQHTPQDFALSPIVAHELYYGAERSQRRAENLARIEALRFPMLEFDREDARHAGEI
RATLAALGTPIGPYDALIGGQARARNLILITRNVREFERVDALSIGTW

Nucleotide

• Length: 387 bp

>T28699 NC_019973:2190115-2190501 [Mesorhizobium australicum WSM2073]
GTGATCTATCTGCTCGATACCAATGCTGTCATCGCCGTCATCAAGGGCGACGCCAGTCTACTCGCGCTTCTGAAGCAGCA
CACGCCGCAGGATTTTGCGCTTTCTCCGATCGTGGCACATGAACTGTATTATGGTGCCGAGAGAAGCCAGAGAAGGGCTG
AAAATCTGGCGCGCATCGAAGCTCTGCGATTCCCGATGCTTGAATTCGATCGGGAGGACGCGAGGCATGCCGGAGAAATT
CGGGCGACGCTTGCAGCATTGGGGACGCCAATCGGCCCCTATGATGCGCTCATTGGCGGACAGGCGCGCGCCAGAAACCT
CATTCTGATCACCCGCAACGTCCGGGAATTCGAGCGCGTCGACGCTCTCTCAATCGGGACTTGGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9403.47 Da
• Isoelectric Point: 4.2797

>AT28699 WP_041163682.1 NC_019973:2189873-2190118 [Mesorhizobium australicum WSM2073]
MDKAKVFWSGRSQAVRLPKEFRFETDEVSIRRHGQTVILEPVVQDWAWLDQVIGSVDEDFAEAALERQQGQDRPALDDIF
E

Nucleotide

• Length: 246 bp

>AT28699 NC_019973:2189873-2190118 [Mesorhizobium australicum WSM2073]
ATGGACAAGGCGAAGGTGTTCTGGTCAGGCCGGTCGCAAGCTGTGCGTCTGCCTAAGGAGTTTCGTTTCGAAACGGATGA
GGTTTCGATTCGTCGCCATGGACAGACTGTCATACTGGAGCCCGTCGTGCAGGATTGGGCGTGGCTTGACCAAGTGATCG
GATCGGTAGATGAGGATTTTGCCGAAGCGGCGCTGGAACGGCAGCAAGGGCAGGATAGACCTGCCTTGGACGACATCTTC
GAGTGA

Structures

Toxin

• AlphaFold DB: L0KGV9