Detailed information of TA system TA028695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5350884..5351509
• Replicon: chromosome
• Accession: NC_019966
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: L0J3Z4
• Locus tag: MYCSM_RS26035
• Protein ID: WP_015309164.1
• Coordinates: 5350884..5351324 (-)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: L0J2Q0
• Locus tag: MYCSM_RS26040
• Protein ID: WP_015309165.1
• Coordinates: 5351330..5351509 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS26005	5346536..5347135	+	600	WP_015309157.1	TetR/AcrR family transcriptional regulator	-
MYCSM_RS26010	5347271..5348803	+	1533	WP_015309159.1	hypothetical protein	-
MYCSM_RS26015	5348800..5349585	+	786	WP_015309160.1	CbbQ/NirQ/NorQ/GpvN family protein	-
MYCSM_RS26025	5349725..5350420	+	696	WP_015309162.1	hypothetical protein	-
MYCSM_RS26030	5350441..5350857	+	417	WP_015309163.1	hypothetical protein	-
MYCSM_RS26035	5350884..5351324	-	441	WP_015309164.1	SRPBCC family protein	Toxin
MYCSM_RS26040	5351330..5351509	-	180	WP_015309165.1	antitoxin	Antitoxin
MYCSM_RS26045	5351519..5353900	-	2382	WP_085976639.1	cation-translocating P-type ATPase	-
MYCSM_RS26050	5353906..5355483	-	1578	WP_015309167.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 15817.57 Da
• Isoelectric Point: 10.3197

>T28695 WP_015309164.1 NC_019966:c5351324-5350884 [Mycobacterium sp. JS623]
MAKLSVSVDVPLPPEKAWACASDLSRYKEWLTIHRVWRSKLPDTLEKGTQLESIVEVKGMPNRIRWTIVHYKPPEAMTLN
GDGKGGVKVKLIGKVKPAGDGSTVTFDVHLGGPALFGPIGMVVAGALKGDIRESLNKFKSVFAPAL

Nucleotide

• Length: 441 bp

>T28695 NC_019966:c5351324-5350884 [Mycobacterium sp. JS623]
ATGGCGAAACTTTCCGTCTCCGTCGACGTTCCATTACCGCCGGAGAAGGCGTGGGCGTGCGCCTCTGACCTGTCCCGGTA
CAAGGAATGGTTGACCATCCACCGGGTATGGCGTTCGAAACTGCCCGACACGCTGGAAAAGGGCACGCAGCTCGAATCCA
TCGTCGAGGTCAAGGGCATGCCGAACCGGATCCGCTGGACCATCGTCCACTACAAGCCTCCAGAGGCAATGACGCTCAAC
GGCGACGGCAAGGGCGGAGTGAAGGTCAAACTCATCGGCAAGGTCAAGCCGGCCGGCGACGGTTCGACGGTCACGTTCGA
CGTACACCTCGGTGGGCCTGCGCTGTTCGGTCCGATCGGCATGGTGGTGGCCGGTGCGCTCAAGGGCGACATCCGCGAAT
CGCTGAACAAGTTCAAGTCGGTATTCGCGCCGGCTTTGTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6304.14 Da
• Isoelectric Point: 9.6591

>AT28695 WP_015309165.1 NC_019966:c5351509-5351330 [Mycobacterium sp. JS623]
MAFLDKVKNLLAHNADKVDTAIDKAGDIVDKKTQGKYSSTVDKVADAAKNAVDKSNPKT

Nucleotide

• Length: 180 bp

>AT28695 NC_019966:c5351509-5351330 [Mycobacterium sp. JS623]
ATGGCATTCCTCGACAAGGTGAAGAATCTGCTGGCCCACAACGCTGACAAGGTGGACACCGCGATCGACAAGGCCGGTGA
CATCGTCGACAAGAAGACGCAGGGCAAGTACAGCTCCACGGTTGACAAGGTGGCCGACGCCGCCAAGAACGCCGTGGACA
AGAGCAACCCGAAGACCTAG

Structures

Toxin

• AlphaFold DB: L0J3Z4

Antitoxin

• AlphaFold DB: L0J2Q0