Detailed information of TA system TA028694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2882220..2882872
• Replicon: chromosome
• Accession: NC_019966
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0IUM6
• Locus tag: MYCSM_RS14135
• Protein ID: WP_015306844.1
• Coordinates: 2882220..2882609 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MYCSM_RS14140
• Protein ID: WP_041314025.1
• Coordinates: 2882606..2882872 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS14110	2878993..2879679	-	687	WP_041314020.1	hypothetical protein	-
MYCSM_RS14115	2879769..2880170	+	402	WP_015306840.1	SRPBCC family protein	-
MYCSM_RS14120	2880167..2880364	-	198	WP_041314023.1	hypothetical protein	-
MYCSM_RS14125	2880452..2880889	+	438	WP_015306842.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MYCSM_RS14130	2880943..2882223	+	1281	WP_015306843.1	threonine ammonia-lyase IlvA	-
MYCSM_RS14135	2882220..2882609	-	390	WP_015306844.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCSM_RS14140	2882606..2882872	-	267	WP_041314025.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MYCSM_RS14145	2882890..2883330	-	441	WP_041312063.1	hypothetical protein	-
MYCSM_RS14150	2883387..2885138	-	1752	WP_015306847.1	malto-oligosyltrehalose trehalohydrolase	-
MYCSM_RS14155	2885131..2887356	-	2226	WP_015306848.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13555.75 Da
• Isoelectric Point: 9.5280

>T28694 WP_015306844.1 NC_019966:c2882609-2882220 [Mycobacterium sp. JS623]
VTIYVDSSALVKIVVYEAESPALARYLASVPDDKRLTAAIARTELLRAVARRGVAVENARKVLARLNFVALTASVLDVAA
TIGPPELRTLDAIHLAAAQVAPELRALVTYDNRLADAARAAGLAVVSPA

Nucleotide

• Length: 390 bp

>T28694 NC_019966:c2882609-2882220 [Mycobacterium sp. JS623]
GTGACTATCTACGTCGACTCGTCGGCGCTTGTGAAGATCGTGGTCTATGAGGCTGAATCACCCGCATTAGCGCGATATCT
GGCGAGCGTGCCGGATGACAAGCGGCTGACCGCTGCGATCGCCCGGACGGAGTTGCTACGGGCTGTTGCTCGTCGGGGTG
TCGCGGTCGAGAACGCACGCAAGGTTCTCGCCAGACTCAATTTCGTCGCGCTCACTGCCAGCGTGCTCGATGTCGCGGCA
ACCATCGGGCCACCTGAGCTTCGTACCCTCGATGCGATTCACCTGGCCGCCGCGCAGGTTGCGCCCGAACTACGTGCGCT
TGTCACGTACGACAACCGCCTGGCCGACGCCGCGAGGGCTGCCGGTCTGGCGGTCGTCTCCCCTGCTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9975.25 Da
• Isoelectric Point: 4.6702

>AT28694 WP_041314025.1 NC_019966:c2882872-2882606 [Mycobacterium sp. JS623]
MERIGLRELRQNASQYIARVARGETVEVTQRGRLVARIVPARPDTWEDMILRGDVIPAESSAALEDVVPISVDFDASSEL
QRQREDRL

Nucleotide

• Length: 267 bp

>AT28694 NC_019966:c2882872-2882606 [Mycobacterium sp. JS623]
ATGGAGCGCATCGGTCTCCGTGAACTGCGACAAAACGCAAGCCAGTACATCGCCCGCGTGGCGCGTGGGGAAACCGTGGA
AGTCACCCAACGCGGCCGATTGGTCGCGCGCATCGTGCCGGCCCGTCCGGACACTTGGGAGGACATGATCCTGCGCGGCG
ACGTCATTCCAGCGGAGTCGTCGGCAGCCCTGGAAGACGTCGTGCCAATCAGCGTCGATTTCGACGCATCCTCAGAACTG
CAGCGGCAACGCGAAGACCGCCTGTGA

Structures

Toxin

• AlphaFold DB: L0IUM6