Detailed information of TA system TA028691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 45103..45683
• Replicon: plasmid pMYCSM03
• Accession: NC_019959
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: doc
• Uniprot ID: L0J714
• Locus tag: MYCSM_RS34485
• Protein ID: WP_015298176.1
• Coordinates: 45303..45683 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MYCSM_RS34480
• Protein ID: WP_041316222.1
• Coordinates: 45103..45306 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS34445	41554..41763	+	210	WP_198345152.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MYCSM_RS37970	41760..41921	-	162	WP_015298169.1	hypothetical protein	-
MYCSM_RS34450	41950..42211	-	262	Protein_49	hypothetical protein	-
MYCSM_RS34455	42566..42919	-	354	WP_015298171.1	hypothetical protein	-
MYCSM_RS34460	42977..43654	-	678	WP_083906460.1	phosphoserine phosphatase SerB	-
MYCSM_RS34465	43771..44196	-	426	WP_015298172.1	hypothetical protein	-
MYCSM_RS34470	44210..44686	-	477	WP_015298173.1	DUF2580 domain-containing protein	-
MYCSM_RS34475	44804..45055	-	252	WP_015298174.1	type II toxin-antitoxin system VapB family antitoxin	-
MYCSM_RS34480	45103..45306	+	204	WP_041316222.1	hypothetical protein	Antitoxin
MYCSM_RS34485	45303..45683	+	381	WP_015298176.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MYCSM_RS37635	45731..46492	-	762	WP_041316259.1	hypothetical protein	-
MYCSM_RS34500	47430..47657	-	228	WP_015298178.1	hypothetical protein	-
MYCSM_RS34505	47810..49561	+	1752	WP_015298179.1	protein kinase	-
MYCSM_RS37645	49591..49773	+	183	WP_157681644.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164114	164114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (51-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13093.73 Da
• Isoelectric Point: 3.9007

>T28691 WP_015298176.1 NC_019959:45303-45683 [Mycobacterium sp. JS623]
VTTFLDRDDVLCAAAAALDGLPEIADYGLLDAAVARPSATVFGLDAYPSLLTKAAALLHSLARNHALVDGNKRTAWAAAW
TFLTINGVELSATYDVDAAEQLMLDVATGVQESVDEIASALEGFAA

Nucleotide

• Length: 381 bp

>T28691 NC_019959:45303-45683 [Mycobacterium sp. JS623]
GTGACGACATTTCTGGACCGCGATGACGTCCTGTGCGCCGCTGCGGCGGCACTCGACGGTCTGCCCGAAATAGCCGACTA
CGGGCTGCTCGATGCAGCGGTCGCACGGCCCAGCGCAACGGTGTTCGGACTAGATGCTTATCCGTCGCTGCTCACGAAGG
CCGCGGCACTACTGCATTCCTTGGCTCGCAACCACGCCCTCGTCGATGGCAATAAGCGCACCGCGTGGGCTGCGGCGTGG
ACGTTCCTGACGATCAACGGCGTGGAGTTGTCTGCAACCTACGACGTCGACGCCGCGGAGCAGCTCATGCTCGACGTAGC
TACGGGTGTACAGGAATCGGTCGACGAAATAGCCAGCGCGCTAGAAGGATTCGCGGCATAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7549.42 Da
• Isoelectric Point: 5.6558

>AT28691 WP_041316222.1 NC_019959:45103-45306 [Mycobacterium sp. JS623]
MPALNVPFDDTEMEALRAAAQQSDQSLKDYVHDAAMQRADRHKEQVAAAAKLVAQRSAELNRRLRDE

Nucleotide

• Length: 204 bp

>AT28691 NC_019959:45103-45306 [Mycobacterium sp. JS623]
ATGCCCGCCCTGAACGTCCCGTTTGACGACACCGAAATGGAAGCGCTGCGTGCTGCTGCCCAGCAGTCGGACCAATCCCT
CAAGGACTACGTGCACGACGCCGCGATGCAGCGGGCTGACCGCCACAAGGAACAGGTAGCAGCGGCGGCGAAACTCGTCG
CGCAGCGCTCGGCAGAGCTGAACCGCAGGCTGCGTGACGAGTGA

Structures

Toxin

• AlphaFold DB: L0J714