Detailed information of TA system TA028690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 30471..31033
• Replicon: plasmid pMYCSM03
• Accession: NC_019959
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0J9G9
• Locus tag: MYCSM_RS34370
• Protein ID: WP_015298153.1
• Coordinates: 30471..30794 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L0J8S6
• Locus tag: MYCSM_RS34375
• Protein ID: WP_015298154.1
• Coordinates: 30791..31033 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS34330	26399..26596	-	198	WP_015298145.1	hypothetical protein	-
MYCSM_RS34340	26778..27791	+	1014	WP_015298146.1	hypothetical protein	-
MYCSM_RS37625	28129..28416	+	288	WP_041316247.1	acyl carrier protein	-
MYCSM_RS34350	28420..28902	+	483	WP_015298148.1	hypothetical protein	-
MYCSM_RS34355	28997..29314	+	318	WP_015298149.1	hypothetical protein	-
MYCSM_RS34360	29301..29705	+	405	WP_015298150.1	hypothetical protein	-
MYCSM_RS34365	29833..30474	-	642	WP_015298152.1	hypothetical protein	-
MYCSM_RS34370	30471..30794	-	324	WP_015298153.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MYCSM_RS34375	30791..31033	-	243	WP_015298154.1	hypothetical protein	Antitoxin
MYCSM_RS34380	31081..31278	-	198	WP_015298155.1	hypothetical protein	-
MYCSM_RS34385	31473..32675	+	1203	WP_041316218.1	hypothetical protein	-
MYCSM_RS34390	32700..32984	-	285	WP_157681635.1	hypothetical protein	-
MYCSM_RS34395	33061..33291	+	231	WP_015298158.1	hypothetical protein	-
MYCSM_RS34400	33292..33555	+	264	WP_015298159.1	hypothetical protein	-
MYCSM_RS34405	33797..34171	-	375	WP_015298160.1	hypothetical protein	-
MYCSM_RS34410	34177..34761	-	585	WP_015298161.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164114	164114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11152.07 Da
• Isoelectric Point: 8.2858

>T28690 WP_015298153.1 NC_019959:c30794-30471 [Mycobacterium sp. JS623]
MTLPARGELWWCEVPGSGARPVVVLSRDAAIPKLRRVLVAPCTTTVRGLASEVVLDPSDDPVPQRAAVNLDLVESVPVTA
LGTRLGRLADSRMAAVCAALVVAVGGR

Nucleotide

• Length: 324 bp

>T28690 NC_019959:c30794-30471 [Mycobacterium sp. JS623]
ATGACGCTGCCGGCGCGCGGTGAGCTGTGGTGGTGCGAGGTCCCCGGCTCGGGTGCCCGGCCGGTGGTGGTGCTCTCCCG
TGATGCGGCGATTCCGAAGCTGAGGCGGGTGCTGGTGGCGCCGTGCACGACGACGGTGCGCGGGTTGGCCAGCGAGGTGG
TGCTCGACCCCAGTGACGATCCGGTACCTCAGCGGGCCGCGGTGAACCTGGATCTGGTCGAGAGCGTGCCGGTGACGGCG
TTGGGAACGCGGTTGGGTCGGCTGGCGGACTCGAGAATGGCGGCGGTGTGCGCCGCGCTGGTGGTGGCGGTGGGCGGCCG
GTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8713.50 Da
• Isoelectric Point: 5.0608

>AT28690 WP_015298154.1 NC_019959:c31033-30791 [Mycobacterium sp. JS623]
MARNRTRVSTTVDADLLASARSLRTGAKDHALFDEALGALLAANRAAEVDASYAAYDEHPADEPDQWGDVADWRRSAGRR

Nucleotide

• Length: 243 bp

>AT28690 NC_019959:c31033-30791 [Mycobacterium sp. JS623]
ATGGCCCGCAATCGCACCCGCGTGAGCACCACGGTCGACGCCGACCTGCTCGCCAGTGCACGCAGCCTGCGGACCGGAGC
CAAGGACCACGCGCTGTTCGACGAGGCCCTGGGCGCGCTGCTGGCCGCCAACCGGGCCGCCGAGGTCGACGCGAGCTACG
CCGCCTACGACGAGCACCCCGCCGACGAGCCCGACCAGTGGGGCGACGTCGCCGACTGGCGCCGATCTGCGGGCCGACGA
TGA

Structures

Toxin

• AlphaFold DB: L0J9G9

Antitoxin

• AlphaFold DB: L0J8S6