Detailed information of TA system TA028689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 248918..249923
• Replicon: plasmid pMYCSM01
• Accession: NC_019957
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L0J8B8
• Locus tag: MYCSM_RS32395
• Protein ID: WP_015297743.1
• Coordinates: 248918..249499 (-)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: L0J7K8
• Locus tag: MYCSM_RS32400
• Protein ID: WP_015297744.1
• Coordinates: 249537..249923 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS32365	244745..245050	-	306	WP_015297736.1	hypothetical protein	-
MYCSM_RS32370	245101..245640	-	540	WP_157681588.1	hypothetical protein	-
MYCSM_RS32375	246019..246597	-	579	WP_015297738.1	helicase DnaB	-
MYCSM_RS32380	246594..246947	-	354	WP_015297739.1	hypothetical protein	-
MYCSM_RS32385	246951..247187	-	237	WP_015297740.1	glutaredoxin-like protein NrdH	-
MYCSM_RS32390	248098..248850	-	753	WP_015297742.1	DUF2127 domain-containing protein	-
MYCSM_RS32395	248918..249499	-	582	WP_015297743.1	zeta toxin family protein	Toxin
MYCSM_RS32400	249537..249923	-	387	WP_015297744.1	DUF3423 domain-containing protein	Antitoxin
MYCSM_RS32405	249986..250204	-	219	WP_157681589.1	hypothetical protein	-
MYCSM_RS32410	250447..252510	+	2064	WP_015297746.1	hypothetical protein	-
MYCSM_RS32415	252514..252714	+	201	WP_015297747.1	hypothetical protein	-
MYCSM_RS32420	252900..253538	+	639	WP_041315897.1	hypothetical protein	-
MYCSM_RS32425	253566..253844	-	279	WP_015297749.1	WhiB family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..394147	394147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (5-124)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21313.40 Da
• Isoelectric Point: 8.1184

>T28689 WP_015297743.1 NC_019957:c249499-248918 [Mycobacterium sp. JS623]
VNRLDLVAGPNGAGKSTFVELTLAPLLKGSVFVNADEIAKVRWPDDPSAHAYEAALIAAETRAKLIELSRPFIAETVFSH
PSKLELIDTAHAAGYTVVLHVLLIPEELAVARVDYRVRAGGHPVAQNKIRQRYHRLWPLVAEAITRCEQTTVYDNSAIKG
PRIVAQFNQGFIVGSPDWPAWTPQPLQSRWPHS

Nucleotide

• Length: 582 bp

>T28689 NC_019957:c249499-248918 [Mycobacterium sp. JS623]
GTGAACCGGCTCGACCTGGTCGCCGGACCCAACGGCGCCGGTAAGTCGACCTTCGTCGAACTGACCCTGGCGCCGCTGCT
GAAAGGCAGCGTGTTCGTCAACGCCGACGAGATCGCCAAAGTGCGCTGGCCCGACGATCCATCGGCCCACGCGTATGAGG
CGGCGCTGATCGCCGCCGAGACCCGCGCGAAGCTGATCGAGCTGAGCCGCCCGTTCATCGCCGAGACGGTGTTCTCCCAT
CCGTCCAAGCTGGAGCTCATCGACACCGCGCACGCCGCCGGCTACACGGTGGTGCTGCATGTGCTGCTCATCCCCGAGGA
GCTGGCGGTGGCCCGCGTGGACTACCGGGTGCGCGCCGGCGGACACCCGGTGGCGCAGAACAAGATTCGCCAGCGCTACC
ACCGGCTGTGGCCACTGGTCGCCGAGGCGATCACCCGCTGCGAACAAACCACCGTCTACGACAACAGCGCCATCAAAGGC
CCACGGATCGTGGCACAGTTCAACCAGGGCTTCATTGTCGGCTCCCCCGACTGGCCGGCCTGGACACCGCAGCCGTTGCA
ATCCCGTTGGCCGCACTCATGA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 13393.74 Da
• Isoelectric Point: 4.9713

>AT28689 WP_015297744.1 NC_019957:c249923-249537 [Mycobacterium sp. JS623]
VAETADRVTRFAADLLDAAAAEGARQSRSAKQQLDHWARVGRAVSSGQTAARRRVEAALAGDLGLAELSEEEGVVFNAEI
SASIEEHLSAGDYGRVLAGRGVTTVALNDKGEIIEHRPDGTSAVVNGR

Nucleotide

• Length: 387 bp

>AT28689 NC_019957:c249923-249537 [Mycobacterium sp. JS623]
GTGGCCGAAACCGCCGACCGGGTGACCCGCTTCGCCGCCGACCTGCTCGACGCCGCGGCGGCCGAGGGTGCGCGCCAGAG
CCGCTCGGCCAAACAACAGCTCGACCATTGGGCCCGGGTGGGTCGGGCGGTCTCCAGCGGGCAGACCGCCGCGCGCCGTC
GAGTGGAGGCCGCGCTGGCCGGCGACCTCGGGCTGGCAGAGCTGAGCGAGGAAGAGGGTGTGGTGTTCAACGCCGAGATC
TCCGCATCGATCGAGGAGCATCTGAGCGCGGGCGACTACGGCCGGGTGCTGGCCGGTCGCGGCGTCACCACCGTTGCCCT
CAACGACAAGGGCGAGATCATCGAGCACCGTCCCGACGGCACCTCGGCCGTGGTGAACGGCCGGTGA

Structures

Toxin

• AlphaFold DB: L0J8B8

Antitoxin

• AlphaFold DB: L0J7K8