Detailed information of TA system TA028688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 182536..183227
• Replicon: plasmid pMYCSM01
• Accession: NC_019957
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0J864
• Locus tag: MYCSM_RS32095
• Protein ID: WP_015297683.1
• Coordinates: 182787..183227 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L0J8Z9
• Locus tag: MYCSM_RS32090
• Protein ID: WP_015297682.1
• Coordinates: 182536..182790 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS32060	178343..178774	-	432	WP_015297676.1	PIN domain nuclease	-
MYCSM_RS32065	178771..178992	-	222	WP_041315748.1	antitoxin	-
MYCSM_RS32070	179184..179432	-	249	WP_015297678.1	hypothetical protein	-
MYCSM_RS32080	180157..180570	-	414	WP_015297680.1	EVE domain-containing protein	-
MYCSM_RS32085	180709..182283	+	1575	WP_015297681.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha/beta	-
MYCSM_RS32090	182536..182790	+	255	WP_015297682.1	hypothetical protein	Antitoxin
MYCSM_RS32095	182787..183227	+	441	WP_015297683.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCSM_RS32100	183567..184604	-	1038	WP_015297684.1	IS110 family transposase	-
MYCSM_RS32105	184695..186137	-	1443	WP_015297685.1	ATPase involved in chromosome partitioning	-
MYCSM_RS32110	186246..186443	-	198	WP_015297686.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..394147	394147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-134)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 15502.73 Da
• Isoelectric Point: 6.6009

>T28688 WP_015297683.1 NC_019957:182787-183227 [Mycobacterium sp. JS623]
VKIVDANVLLYAVNSAAEHHQASRRWLDVALSGADTVGLAWVPLLAFVRLSTKIGLFPSPLHPGAAMQQVEDWCGAPAAV
VIGPTPRHPQLLSGLLAHVGTGGNLVNDAHLAALALEHRGGIVSYDSDFGRFDGVRWDTPDALMKQ

Nucleotide

• Length: 441 bp

>T28688 NC_019957:182787-183227 [Mycobacterium sp. JS623]
GTGAAGATCGTCGACGCCAATGTCCTGCTTTACGCGGTCAATTCGGCCGCCGAACACCACCAGGCCAGCAGGCGCTGGCT
CGACGTTGCACTGTCGGGCGCCGACACGGTCGGGTTGGCGTGGGTGCCGCTGCTGGCGTTCGTGCGGCTGAGCACCAAGA
TCGGACTCTTCCCGTCCCCGCTGCACCCCGGCGCAGCCATGCAGCAGGTCGAGGACTGGTGCGGCGCGCCAGCCGCGGTC
GTGATCGGTCCGACCCCGCGCCACCCCCAACTGCTCAGCGGGCTGCTGGCCCACGTCGGGACGGGCGGAAACCTCGTCAA
CGACGCACACCTGGCAGCTCTGGCGCTGGAGCACCGTGGCGGGATCGTCAGCTATGACAGCGATTTCGGCCGTTTCGACG
GCGTGCGCTGGGATACGCCCGATGCGCTGATGAAGCAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9382.70 Da
• Isoelectric Point: 11.5471

>AT28688 WP_015297682.1 NC_019957:182536-182790 [Mycobacterium sp. JS623]
MRTTVTLDADTLALVRQRMRARGVSFKQALNDAIRDGAHGRPSPTRFVTKTADLGVPNINLDRALQLAAELEDEELLRRQ
RRGA

Nucleotide

• Length: 255 bp

>AT28688 NC_019957:182536-182790 [Mycobacterium sp. JS623]
ATGCGCACGACGGTGACGCTCGACGCCGACACGCTGGCGTTGGTGCGCCAGCGGATGCGTGCGCGCGGCGTGTCCTTCAA
GCAGGCCCTCAACGACGCGATTCGCGACGGAGCCCACGGCCGACCGTCTCCGACACGGTTCGTCACCAAGACCGCCGACC
TCGGCGTTCCGAACATCAATCTCGACCGTGCCCTGCAGCTTGCCGCCGAACTTGAAGACGAGGAGCTGCTGCGCCGGCAG
CGCCGCGGCGCGTGA

Structures

Toxin

• AlphaFold DB: L0J864

Antitoxin

• AlphaFold DB: L0J8Z9