Detailed information of TA system TA028687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 178343..178992
• Replicon: plasmid pMYCSM01
• Accession: NC_019957
• Organism: Mycobacterium smegmatis JS623

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0J5H2
• Locus tag: MYCSM_RS32060
• Protein ID: WP_015297676.1
• Coordinates: 178343..178774 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MYCSM_RS32065
• Protein ID: WP_041315748.1
• Coordinates: 178771..178992 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCSM_RS32050	173820..176030	+	2211	WP_157681567.1	LuxR family transcriptional regulator	-
MYCSM_RS32055	177190..178002	+	813	WP_015297675.1	ZIP family metal transporter	-
MYCSM_RS32060	178343..178774	-	432	WP_015297676.1	PIN domain nuclease	Toxin
MYCSM_RS32065	178771..178992	-	222	WP_041315748.1	antitoxin	Antitoxin
MYCSM_RS32070	179184..179432	-	249	WP_015297678.1	hypothetical protein	-
MYCSM_RS32080	180157..180570	-	414	WP_015297680.1	EVE domain-containing protein	-
MYCSM_RS32085	180709..182283	+	1575	WP_015297681.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha/beta	-
MYCSM_RS32090	182536..182790	+	255	WP_015297682.1	hypothetical protein	-
MYCSM_RS32095	182787..183227	+	441	WP_015297683.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..394147	394147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15679.63 Da
• Isoelectric Point: 4.6700

>T28687 WP_015297676.1 NC_019957:c178774-178343 [Mycobacterium sp. JS623]
VSEPEWLIDKSALVRLAHSDDPDLWANRIERGLVHISNVTRLEIGYSAQSGEVARREFRESPLAAMSVEYLTPTIEDRAS
EVQLLLADRGQHRGPSIPDLIVAATAELAGLTILHVDKDFDTITAITGQPTTRLAFTHAASPE

Nucleotide

• Length: 432 bp

>T28687 NC_019957:c178774-178343 [Mycobacterium sp. JS623]
GTGAGCGAACCGGAGTGGCTCATCGACAAGTCCGCCCTGGTCCGACTTGCACACAGCGACGACCCGGACCTGTGGGCCAA
CCGCATCGAGCGCGGACTGGTCCACATCAGTAACGTGACGCGGCTGGAAATCGGCTATTCGGCTCAATCCGGTGAAGTCG
CGCGCCGCGAGTTCCGTGAATCACCCTTGGCGGCAATGTCTGTCGAATATTTGACACCGACCATTGAGGATCGCGCGTCG
GAAGTCCAACTCCTGCTCGCAGACCGGGGTCAGCACCGCGGTCCGTCAATTCCCGACCTCATCGTCGCGGCCACCGCCGA
GCTCGCCGGGTTGACCATCCTGCACGTCGACAAAGACTTCGACACCATCACCGCGATCACAGGACAACCCACAACGCGCC
TAGCCTTCACCCACGCGGCCAGTCCGGAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8221.36 Da
• Isoelectric Point: 4.6182

>AT28687 WP_041315748.1 NC_019957:c178992-178771 [Mycobacterium sp. JS623]
VSDVLIRDIPDDVLAGLDARAAELGLSRVEYIRRRLAQDARMTRVAVTREDLKRLGQAVSGLADEELMREAWE

Nucleotide

• Length: 222 bp

>AT28687 NC_019957:c178992-178771 [Mycobacterium sp. JS623]
GTGAGTGACGTGTTGATCCGAGACATACCCGATGATGTGCTGGCGGGTCTCGACGCGCGAGCCGCTGAGTTGGGCTTATC
GCGCGTTGAGTACATTCGCCGCCGCCTCGCCCAAGATGCCCGGATGACGCGCGTTGCGGTGACTCGTGAGGACCTCAAGC
GGCTCGGACAGGCCGTTTCCGGCCTCGCTGACGAGGAGTTGATGCGCGAGGCGTGGGAGTGA

Structures

Toxin

• AlphaFold DB: L0J5H2