286803

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 33726..34834 Replicon plasmid 6
Accession NZ_LR134110
Organism Enterococcus faecium isolate E6043

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag ELZ65_RS15885 Protein ID WP_000233000.1
Coordinates 33965..34834 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag ELZ65_RS15880 Protein ID WP_000205227.1
Coordinates 33726..33950 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELZ65_RS16520 29315..29485 + 171 WP_000713595.1 hypothetical protein -
ELZ65_RS15855 29499..30101 + 603 WP_001062586.1 recombinase family protein -
ELZ65_RS15860 30117..30794 + 678 Protein_35 DNA topoisomerase -
ELZ65_RS15865 31306..31602 + 297 WP_002360685.1 hypothetical protein -
ELZ65_RS15870 31603..32019 + 417 WP_000323438.1 recombinase -
ELZ65_RS15875 32021..33583 + 1563 WP_000136908.1 recombinase family protein -
ELZ65_RS15880 33726..33950 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
ELZ65_RS15885 33965..34834 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
ELZ65_RS15890 34815..35549 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
ELZ65_RS15895 35582..36445 + 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
ELZ65_RS15900 36489..37016 + 528 WP_002294507.1 adenine phosphoribosyltransferase -
ELZ65_RS15905 37244..38563 + 1320 WP_002338419.1 IS1380-like element ISSsu5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cat(pC194) / ant(6)-Ia - 1..42954 42954
- flank IS/Tn ant(6)-Ia - 35582..38563 2981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T286803 WP_000233000.1 NZ_LR134110:33965-34834 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT286803 WP_000205227.1 NZ_LR134110:33726-33950 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References