Detailed information of TA system TA028677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3015591..3016171
• Replicon: chromosome
• Accession: NC_019940
• Organism: Thioflavicoccus mobilis 8321

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0GZP0
• Locus tag: THIMO_RS13005
• Protein ID: WP_015281568.1
• Coordinates: 3015591..3015962 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L0H044
• Locus tag: THIMO_RS13010
• Protein ID: WP_015281569.1
• Coordinates: 3015950..3016171 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIMO_RS12990	3013455..3013877	-	423	WP_015281564.1	putative toxin-antitoxin system toxin component, PIN family	-
THIMO_RS12995	3013861..3014103	-	243	WP_015281565.1	hypothetical protein	-
THIMO_RS13000	3014681..3014983	-	303	WP_015281567.1	nucleotidyltransferase family protein	-
THIMO_RS13005	3015591..3015962	-	372	WP_015281568.1	type II toxin-antitoxin system VapC family toxin	Toxin
THIMO_RS13010	3015950..3016171	-	222	WP_015281569.1	ribbon-helix-helix domain-containing protein	Antitoxin
THIMO_RS13015	3017183..3018127	-	945	WP_015281571.1	ferritin family protein	-
THIMO_RS20270	3019024..3019377	-	354	WP_015281572.1	DUF5615 family PIN-like protein	-
THIMO_RS19235	3019374..3019626	-	253	Protein_2657	DUF433 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3004749..3019626	14877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-40)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13872.18 Da
• Isoelectric Point: 8.9882

>T28677 WP_015281568.1 NC_019940:c3015962-3015591 [Thioflavicoccus mobilis 8321]
MGPVKALFDTNILIDYLNGVVAARDEIGRYQRPLISLINWMEVLTGTPAEHAQPVRAFLRRFECVGIDAEIAERAVNLRQ
AQRLKLPDAIIRATAMERSALLVTRNTKDFLADEPGIRIPYKL

Nucleotide

• Length: 372 bp

>T28677 NC_019940:c3015962-3015591 [Thioflavicoccus mobilis 8321]
ATGGGACCGGTGAAGGCGCTGTTCGACACCAACATCCTGATCGACTACCTGAACGGTGTGGTCGCGGCCCGCGACGAGAT
CGGCCGCTACCAGAGGCCCCTGATCAGTCTGATCAATTGGATGGAGGTCTTGACGGGTACGCCAGCGGAGCACGCCCAGC
CGGTCCGTGCCTTCTTGCGCCGCTTCGAGTGCGTCGGCATCGACGCAGAAATCGCCGAGCGTGCGGTCAATCTCAGGCAG
GCGCAGCGCCTCAAGCTTCCTGATGCGATCATCCGTGCCACCGCAATGGAGCGCAGTGCCCTGCTGGTGACACGCAATAC
CAAGGATTTCCTTGCGGACGAGCCTGGCATCCGTATCCCCTATAAGTTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8476.41 Da
• Isoelectric Point: 4.6413

>AT28677 WP_015281569.1 NC_019940:c3016171-3015950 [Thioflavicoccus mobilis 8321]
MRTVIDLPDDQIAPLKDLAESLNVSRAELIRRAVTDFLRRHEVPTEDAAFGIWRGHDEDGVAYQERARSEWDR

Nucleotide

• Length: 222 bp

>AT28677 NC_019940:c3016171-3015950 [Thioflavicoccus mobilis 8321]
ATGCGCACAGTTATCGACCTGCCGGACGACCAAATCGCGCCGCTCAAGGATCTGGCGGAGTCCCTCAATGTCTCGCGCGC
CGAGCTGATTCGGCGTGCGGTGACTGACTTCCTGCGCCGGCACGAGGTGCCGACGGAAGATGCAGCGTTCGGCATCTGGA
GGGGGCATGATGAAGACGGTGTCGCCTACCAAGAGCGCGCGCGCTCGGAATGGGACCGGTGA

Structures

Toxin

• AlphaFold DB: L0GZP0

Antitoxin

• AlphaFold DB: L0H044