Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 987504..988158 Replicon chromosome
Accession NZ_LR134092
Organism Escherichia coli strain NCTC10444

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EL041_RS04845 Protein ID WP_000244777.1
Coordinates 987751..988158 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EL041_RS04840 Protein ID WP_000354046.1
Coordinates 987504..987770 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL041_RS04820 983634..983945 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EL041_RS04825 984109..984768 + 660 WP_000250270.1 hemolysin III family protein -
EL041_RS04830 984855..986203 + 1349 WP_100245061.1 IS3 family transposase -
EL041_RS04835 986281..987261 - 981 WP_000886100.1 tRNA-modifying protein YgfZ -
EL041_RS04840 987504..987770 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EL041_RS04845 987751..988158 + 408 WP_000244777.1 toxin CptA Toxin
EL041_RS04850 988198..988719 - 522 WP_001055874.1 flavodoxin FldB -
EL041_RS04855 988831..989727 + 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
EL041_RS04860 989752..990462 + 711 WP_000715218.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EL041_RS04865 990468..992201 + 1734 WP_000813236.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T286760 WP_000244777.1 NZ_LR134092:987751-988158 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT286760 WP_000354046.1 NZ_LR134092:987504-987770 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References