Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 353424..354224 Replicon chromosome
Accession NZ_LR134092
Organism Escherichia coli strain NCTC10444

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag EL041_RS01675 Protein ID WP_000342447.1
Coordinates 353697..354224 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag EL041_RS01670 Protein ID WP_001277109.1
Coordinates 353424..353690 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL041_RS01650 349082..349750 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
EL041_RS01655 349743..350801 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
EL041_RS01660 351046..351900 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EL041_RS01665 352171..353274 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EL041_RS01670 353424..353690 + 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
EL041_RS01675 353697..354224 + 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
EL041_RS01680 354221..354604 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
EL041_RS01685 355028..356137 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EL041_RS01690 356185..357111 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EL041_RS01695 357108..358385 + 1278 WP_000803778.1 branched chain amino acid ABC transporter permease LivM -
EL041_RS01700 358382..359149 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T286757 WP_000342447.1 NZ_LR134092:353697-354224 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT286757 WP_001277109.1 NZ_LR134092:353424-353690 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References