Detailed information of TA system TA028668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 18130..18823
• Replicon: plasmid pPC9
• Accession: NC_019906
• Organism: Pseudomonas putida HB3267

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: B479_RS26870
• Protein ID: WP_003151133.1
• Coordinates: 18446..18823 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: B479_RS26865
• Protein ID: WP_001172026.1
• Coordinates: 18130..18465 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B479_RS26830	13790..14593	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
B479_RS26835	14659..14979	-	321	Protein_17	recombinase family protein	-
B479_RS26840	15033..15737	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B479_RS28300	15894..16709	-	816	WP_000018326.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
B479_RS26845	16860..17033	+	174	Protein_20	IS6 family transposase	-
B479_RS26850	17073..17414	-	342	WP_025999701.1	hypothetical protein	-
B479_RS26855	17430..17756	-	327	WP_000091614.1	hypothetical protein	-
B479_RS26860	17780..18115	-	336	WP_000741275.1	hypothetical protein	-
B479_RS26865	18130..18465	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
B479_RS26870	18446..18823	-	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B479_RS26875	19015..19617	+	603	WP_010465829.1	recombinase family protein	-
B479_RS26880	19601..22630	+	3030	WP_010799689.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / qacE / cmlA1 / ant(3'')-Ia / blaVIM-1 / blaAER-1 / ant(2'')-Ia	-	1..80360	80360
	-	inside	IScluster/Tn	sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia	-	1740..22630	20890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T28668 WP_003151133.1 NC_019906:c18823-18446 [Pseudomonas putida HB3267]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T28668 NC_019906:c18823-18446 [Pseudomonas putida HB3267]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT28668 WP_001172026.1 NC_019906:c18465-18130 [Pseudomonas putida HB3267]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT28668 NC_019906:c18465-18130 [Pseudomonas putida HB3267]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7