Detailed information of TA system TA028667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3883017..3884056
• Replicon: chromosome
• Accession: NC_019905
• Organism: Pseudomonas putida HB3267

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A2N5BP56
• Locus tag: B479_RS17780
• Protein ID: WP_015271072.1
• Coordinates: 3883481..3884056 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A2N5BP88
• Locus tag: B479_RS17775
• Protein ID: WP_015271071.1
• Coordinates: 3883017..3883484 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B479_RS17755	3879081..3880100	+	1020	WP_013973404.1	ABC transporter permease	-
B479_RS17760	3880102..3881715	+	1614	WP_015271068.1	ABC transporter ATP-binding protein	-
B479_RS17765	3882173..3882415	+	243	WP_015271069.1	hypothetical protein	-
B479_RS17770	3882572..3882835	+	264	WP_015271070.1	DUF3077 domain-containing protein	-
B479_RS17775	3883017..3883484	+	468	WP_015271071.1	helix-turn-helix domain-containing protein	Antitoxin
B479_RS17780	3883481..3884056	+	576	WP_015271072.1	PIN domain-containing protein	Toxin
B479_RS17785	3884271..3887201	+	2931	WP_015271073.1	aminotransferase	-
B479_RS17790	3887331..3888236	+	906	WP_013973410.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3861946..3887201	25255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• SinI-like, DNA-binding domain (84-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21970.23 Da
• Isoelectric Point: 5.0798

>T28667 WP_015271072.1 NC_019905:3883481-3884056 [Pseudomonas putida HB3267]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSTKIHDEWKRNLLLNRPDLTLEQVDRTSRRMDAAVPDALVTDY
ESLVEGLDLPDKDDRHVLAAAIKCNASVIVTFNLKDFPETALDVFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRMSLKN
PPHSVQQYLDRLLQQKLPESVKLLSEFKFLL

Nucleotide

• Length: 576 bp

>T28667 NC_019905:3883481-3884056 [Pseudomonas putida HB3267]
ATGAAGCACTCGCCCTTCACGGCCATTTACGACGCGAACGTTCTTTATCCTGCCCCACTCCGTGATTTCCTGATGAACCT
TGCATTGACAGGTATTTACCGAGCACGTTGGTCAACGAAGATTCATGACGAGTGGAAACGCAATTTATTGCTCAACCGGC
CTGACCTCACCCTCGAGCAAGTAGATCGGACGTCACGGCGAATGGACGCTGCTGTACCTGACGCACTCGTAACTGACTAC
GAGTCGCTGGTAGAAGGGCTGGACTTGCCAGATAAGGATGATCGACATGTCCTCGCTGCTGCAATCAAGTGCAACGCTTC
CGTCATCGTCACCTTCAACCTGAAGGATTTCCCCGAAACGGCTCTCGACGTTTTCGATATCGAGCCCTTACACCCGGATG
ACTTCATCGCCGACCTGTGGGACCTGGACAAGGCAGCAGTACTTGAGGCTGCCCAGCGGCAGCGAATGTCCTTGAAAAAC
CCACCTCACAGCGTGCAGCAGTATCTCGACAGACTGCTTCAGCAAAAGCTTCCCGAGTCGGTGAAGTTGCTTTCAGAATT
CAAATTCCTCCTCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17366.86 Da
• Isoelectric Point: 5.3408

>AT28667 WP_015271071.1 NC_019905:3883017-3883484 [Pseudomonas putida HB3267]
MTTADLSRKMLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPYLVKLLDESVIPHTKTGRHRRVKFSDLVAYKDKRDEVSRAAMDALAAEAQELRMGYE

Nucleotide

• Length: 468 bp

>AT28667 NC_019905:3883017-3883484 [Pseudomonas putida HB3267]
ATGACCACCGCCGACCTCTCTCGCAAGATGCTTCCCGATGAGAAAGAGATTGCCGTGGCAGTCGAATCGAGCCGAACGCT
AGCAGCCTTCCTCTCGACAAAATCAGATACCCAACGGATTGAGCTGCTCAATGAAGAGAATCAGCGCCAGGTTGTTGAAG
TACCTACCTTCGCGCTGCGTCTGTTTGGCGAGATTCTCAGCGAGCTGGCTCTGGGGAACTCGGTCAAAGTCGTTCCAATC
CACGCCGAATTGACTACCCAAGAGGCAGCAGACCTACTCAACGTCTCTCGGCCGTACTTGGTAAAACTGCTAGACGAATC
TGTGATTCCTCACACAAAAACGGGTCGCCATCGTCGGGTCAAATTCTCCGACCTGGTTGCCTACAAGGACAAACGTGACG
AGGTAAGCCGTGCCGCAATGGACGCGCTGGCAGCCGAAGCCCAGGAGTTACGGATGGGGTACGAATGA

Structures

Toxin

• AlphaFold DB: A0A2N5BP56

Antitoxin

• AlphaFold DB: A0A2N5BP88