Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2351125..2351651 Replicon chromosome
Accession NZ_LR134080
Organism Escherichia coli strain NCTC9082

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EL023_RS11585 Protein ID WP_000323025.1
Coordinates 2351364..2351651 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EL023_RS11580 Protein ID WP_000534858.1
Coordinates 2351125..2351364 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL023_RS11550 2346487..2346843 + 357 WP_001310834.1 class I SAM-dependent methyltransferase -
EL023_RS24900 2347454..2347741 + 288 Protein_2214 hypothetical protein -
EL023_RS11565 2348510..2349823 + 1314 WP_000589005.1 ISNCY family transposase -
EL023_RS11570 2350260..2350592 - 333 WP_001301033.1 protein FlxA -
EL023_RS11575 2350795..2351100 - 306 WP_001326990.1 hypothetical protein -
EL023_RS11580 2351125..2351364 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EL023_RS11585 2351364..2351651 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EL023_RS11590 2351723..2351878 + 156 WP_088376227.1 type I toxin-antitoxin system toxin HokD -
EL023_RS11595 2352095..2352346 + 252 WP_000980994.1 hypothetical protein -
EL023_RS11600 2352413..2352691 + 279 WP_012304870.1 hypothetical protein -
EL023_RS11605 2352693..2353742 + 1050 WP_001265198.1 DUF968 domain-containing protein -
EL023_RS11610 2353756..2354508 + 753 WP_001047135.1 antitermination protein -
EL023_RS11615 2354786..2354875 - 90 WP_120795389.1 hypothetical protein -
EL023_RS11620 2354930..2355142 - 213 WP_000087756.1 cold shock-like protein CspF -
EL023_RS11625 2355443..2355658 + 216 WP_000066484.1 cold shock-like protein CspB -
EL023_RS25030 2356022..2356192 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
EL023_RS11630 2356412..2356627 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sitABCD fimA / espR1 / rhs/PAAR / vgrG/tssI 2257138..2693419 436281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T286659 WP_000323025.1 NZ_LR134080:2351364-2351651 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT286659 WP_000534858.1 NZ_LR134080:2351125-2351364 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References