Detailed information of TA system TA028665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2369758..2370274
• Replicon: chromosome
• Accession: NC_019905
• Organism: Pseudomonas putida HB3267

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: B479_RS10995
• Protein ID: WP_003259987.1
• Coordinates: 2369993..2370274 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: B479_RS10990
• Protein ID: WP_013972043.1
• Coordinates: 2369758..2370003 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B479_RS10965	2365170..2365649	-	480	WP_015269934.1	transposase	-
B479_RS10970	2365906..2366448	-	543	WP_015269935.1	WYL domain-containing protein	-
B479_RS10980	2367184..2368377	+	1194	WP_015269936.1	MFS transporter	-
B479_RS10985	2368408..2369514	+	1107	WP_015269937.1	alkene reductase	-
B479_RS10990	2369758..2370003	+	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B479_RS10995	2369993..2370274	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B479_RS11000	2370290..2370922	-	633	WP_015269938.1	BRCT domain-containing protein	-
B479_RS11005	2370939..2371334	-	396	WP_023662326.1	hypothetical protein	-
B479_RS11010	2371423..2372331	-	909	WP_015269940.1	LysR family transcriptional regulator	-
B479_RS11015	2372623..2373927	+	1305	WP_013972046.1	MHS family MFS transporter	-
B479_RS11020	2373958..2375199	+	1242	WP_015269941.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T28665 WP_003259987.1 NC_019905:2369993-2370274 [Pseudomonas putida HB3267]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T28665 NC_019905:2369993-2370274 [Pseudomonas putida HB3267]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGTATCGTTGTAGTGGTGGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT28665 WP_013972043.1 NC_019905:2369758-2370003 [Pseudomonas putida HB3267]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT28665 NC_019905:2369758-2370003 [Pseudomonas putida HB3267]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AACGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTATCCCTGGATGAC
CTATAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6