Detailed information of TA system TA028663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1116153..1116778
• Replicon: chromosome
• Accession: NC_019903
• Organism: Desulfitobacterium dichloroeliminans LMG P-21439

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: L0F6E1
• Locus tag: DESDI_RS05160
• Protein ID: WP_015261581.1
• Coordinates: 1116428..1116778 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L0F437
• Locus tag: DESDI_RS05155
• Protein ID: WP_015261580.1
• Coordinates: 1116153..1116431 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DESDI_RS05135	1111871..1112887	+	1017	WP_083879887.1	2-hydroxyacid dehydrogenase family protein	-
DESDI_RS05140	1113000..1113524	-	525	WP_015261577.1	gamma carbonic anhydrase family protein	-
DESDI_RS05145	1113710..1115119	+	1410	WP_015261578.1	Mur ligase family protein	-
DESDI_RS05150	1115116..1115874	+	759	WP_015261579.1	glutamine amidotransferase	-
DESDI_RS05155	1116153..1116431	+	279	WP_015261580.1	ribbon-helix-helix protein, CopG family	Antitoxin
DESDI_RS05160	1116428..1116778	+	351	WP_015261581.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DESDI_RS05165	1116940..1117659	+	720	WP_041219743.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DESDI_RS05170	1117748..1117945	-	198	WP_015261583.1	alpha/beta-type small acid-soluble spore protein	-
DESDI_RS05175	1118116..1120344	-	2229	WP_015261584.1	sigma-54-dependent Fis family transcriptional regulator	-
DESDI_RS05180	1120689..1121264	+	576	WP_015261585.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-39)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12852.88 Da
• Isoelectric Point: 5.8945

>T28663 WP_015261581.1 NC_019903:1116428-1116778 [Desulfitobacterium dichloroeliminans LMG P-21439]
MIIKRGEIYYAELNPVIGSEQGGTRPVLVVQNDIGNQFSPTTIIAAITSQIAKAKLPTHVEVRARRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDQAIEISLGLTDI

Nucleotide

• Length: 351 bp

>T28663 NC_019903:1116428-1116778 [Desulfitobacterium dichloroeliminans LMG P-21439]
ATGATTATAAAACGCGGGGAAATTTATTATGCTGAACTAAACCCCGTCATCGGATCCGAGCAAGGCGGTACGCGTCCTGT
TTTGGTGGTCCAAAATGATATCGGAAATCAATTCAGTCCGACGACTATTATTGCCGCGATTACATCGCAGATTGCTAAAG
CAAAATTGCCGACTCATGTAGAAGTACGGGCAAGACGTAGCGGCTTGGAACGGGATTCTGTGATTCTGACGGAGCAAATC
CGCACCATAGATAAAAGCCGTTTGAAAGAGAAGGTCGCCGTGCTCGATGAAGAAGTGATGCTGAGAGTAGACCAAGCTAT
CGAAATCAGCTTGGGACTAACAGATATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10773.19 Da
• Isoelectric Point: 4.7672

>AT28663 WP_015261580.1 NC_019903:1116153-1116431 [Desulfitobacterium dichloroeliminans LMG P-21439]
VAESKRIMISLPENLLAEVDDVVTLENRNRSEFIREAINSILHERRKRGIREQMRKGYQEMAQLNLAIARELFLAEDEVQ
PTFEDSNAECQR

Nucleotide

• Length: 279 bp

>AT28663 NC_019903:1116153-1116431 [Desulfitobacterium dichloroeliminans LMG P-21439]
GTGGCTGAGAGTAAGCGGATAATGATCAGTTTACCGGAGAATTTGCTTGCGGAAGTGGATGATGTCGTTACATTAGAGAA
TAGAAATCGTAGCGAATTTATACGCGAAGCAATTAATAGTATTCTTCACGAACGCCGGAAACGGGGAATCCGCGAGCAAA
TGCGTAAAGGATATCAGGAAATGGCTCAATTAAACTTAGCCATTGCAAGAGAATTATTCCTTGCCGAGGATGAAGTTCAA
CCGACATTTGAAGATTCTAATGCGGAGTGTCAGAGATGA

Structures

Toxin

• AlphaFold DB: L0F6E1

Antitoxin

• AlphaFold DB: L0F437