Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1001196..1001850 Replicon chromosome
Accession NZ_LR134079
Organism Escherichia coli strain NCTC9112

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EL053_RS04950 Protein ID WP_000244777.1
Coordinates 1001443..1001850 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EL053_RS04945 Protein ID WP_000354046.1
Coordinates 1001196..1001462 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL053_RS04925 997326..997637 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EL053_RS04930 997801..998460 + 660 WP_000250270.1 hemolysin III family protein -
EL053_RS04935 998547..999895 + 1349 WP_100245061.1 IS3 family transposase -
EL053_RS04940 999973..1000953 - 981 WP_000886100.1 tRNA-modifying protein YgfZ -
EL053_RS04945 1001196..1001462 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EL053_RS04950 1001443..1001850 + 408 WP_000244777.1 toxin CptA Toxin
EL053_RS04955 1001890..1002411 - 522 WP_001055874.1 flavodoxin FldB -
EL053_RS04960 1002523..1003419 + 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
EL053_RS04965 1003444..1004154 + 711 WP_000715218.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EL053_RS04970 1004160..1005893 + 1734 WP_000813236.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T286628 WP_000244777.1 NZ_LR134079:1001443-1001850 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT286628 WP_000354046.1 NZ_LR134079:1001196-1001462 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References