Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 335102..335902 Replicon chromosome
Accession NZ_LR134079
Organism Escherichia coli strain NCTC9112

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag EL053_RS01545 Protein ID WP_000342447.1
Coordinates 335375..335902 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag EL053_RS01540 Protein ID WP_001277109.1
Coordinates 335102..335368 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL053_RS01520 330760..331428 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
EL053_RS01525 331421..332479 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
EL053_RS01530 332724..333578 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EL053_RS01535 333849..334952 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EL053_RS01540 335102..335368 + 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
EL053_RS01545 335375..335902 + 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
EL053_RS01550 335899..336282 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
EL053_RS01555 336706..337815 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EL053_RS01560 337863..338789 + 927 WP_061350008.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EL053_RS01565 338786..340063 + 1278 WP_000803778.1 branched chain amino acid ABC transporter permease LivM -
EL053_RS01570 340060..340827 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T286625 WP_000342447.1 NZ_LR134079:335375-335902 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT286625 WP_001277109.1 NZ_LR134079:335102-335368 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References