Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3911030..3911606 Replicon chromosome
Accession NZ_LR134075
Organism Escherichia coli strain NCTC9084

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL019_RS19095 Protein ID WP_001297643.1
Coordinates 3911319..3911606 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EL019_RS19090 Protein ID WP_000063156.1
Coordinates 3911030..3911332 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL019_RS19075 3907626..3909776 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EL019_RS19080 3909826..3910029 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL019_RS19085 3910040..3910996 + 957 WP_001297640.1 GTPase -
EL019_RS19090 3911030..3911332 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EL019_RS19095 3911319..3911606 - 288 WP_001297643.1 BrnT family toxin Toxin
EL019_RS19100 3911805..3912502 - 698 Protein_3699 IS1 family transposase -
EL019_RS19105 3912559..3912654 + 96 Protein_3700 dienelactone hydrolase family protein -
EL019_RS19110 3912800..3914032 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EL019_RS19115 3914073..3915353 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T286621 WP_001297643.1 NZ_LR134075:c3911606-3911319 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT286621 WP_000063156.1 NZ_LR134075:c3911332-3911030 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References