Detailed information of TA system TA028661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 3994969..3995534
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L0E2J6
• Locus tag: TVNIR_RS18260
• Protein ID: WP_015260569.1
• Coordinates: 3995190..3995534 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TVNIR_RS18255
• Protein ID: WP_043739929.1
• Coordinates: 3994969..3995193 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS18235	3990261..3990833	+	573	WP_015260564.1	helix-turn-helix domain-containing protein	-
TVNIR_RS18240	3991212..3992174	+	963	WP_015260565.1	SDR family oxidoreductase	-
TVNIR_RS18245	3992174..3993157	+	984	WP_015260566.1	UDP-glucose 4-epimerase GalE	-
TVNIR_RS18250	3993572..3994585	+	1014	WP_015260567.1	hypothetical protein	-
TVNIR_RS18255	3994969..3995193	+	225	WP_043739929.1	DUF433 domain-containing protein	Antitoxin
TVNIR_RS18260	3995190..3995534	+	345	WP_015260569.1	DUF5615 family PIN-like protein	Toxin
TVNIR_RS19445	3995753..3996010	+	258	Protein_3668	ABC transporter permease	-
TVNIR_RS18270	3996504..3996743	+	240	WP_015260572.1	type II toxin-antitoxin system VapB family antitoxin	-
TVNIR_RS18275	3996740..3997135	+	396	WP_015260573.1	type II toxin-antitoxin system VapC family toxin	-
TVNIR_RS18280	3997231..3997509	+	279	WP_015260574.1	BrnT family toxin	-
TVNIR_RS18285	3997484..3997759	+	276	WP_015260575.1	BrnA antitoxin family protein	-
TVNIR_RS18290	3998012..3998222	+	211	Protein_3673	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
TVNIR_RS18295	3998219..3998599	+	381	WP_015260577.1	type II toxin-antitoxin system VapC family toxin	-
TVNIR_RS19930	3998802..3999313	+	512	Protein_3675	hypothetical protein	-
TVNIR_RS18310	3999827..4000126	+	300	WP_043739932.1	nucleotidyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3992174..4001768	9594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-78)

Antitoxin

• Protein of unknown function DUF433 (8-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12805.73 Da
• Isoelectric Point: 4.6437

>T28661 WP_015260569.1 NC_019902:3995190-3995534 [Thioalkalivibrio nitratireducens DSM 14787]
MRIWVDAQLSPHLAPWLTERFGIEAYSVRRLGYRDATDEAIFAAAREADAVVMTKDRDFLNLLDAQGPPPRVIWITMGNT
SNARMRAVLDSVFLEALASLRQGEAMVEITDLMP

Nucleotide

• Length: 345 bp

>T28661 NC_019902:3995190-3995534 [Thioalkalivibrio nitratireducens DSM 14787]
ATGAGAATCTGGGTTGACGCCCAACTCTCTCCCCACCTCGCCCCCTGGCTCACCGAGCGCTTCGGTATCGAAGCATACTC
CGTCCGCCGGCTGGGATACCGCGACGCCACCGACGAAGCCATCTTCGCGGCCGCGCGCGAAGCCGATGCGGTGGTGATGA
CCAAGGATCGCGATTTTCTGAACCTCCTCGATGCACAGGGACCTCCACCAAGGGTGATCTGGATCACCATGGGAAACACC
TCCAACGCCCGCATGCGGGCCGTGTTGGACTCAGTATTCCTCGAGGCACTTGCTTCGCTGCGCCAAGGCGAGGCGATGGT
GGAGATCACTGATCTCATGCCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8068.40 Da
• Isoelectric Point: 5.7390

>AT28661 WP_043739929.1 NC_019902:3994969-3995193 [Thioalkalivibrio nitratireducens DSM 14787]
MNDLLRRITVNPAQCGGRPCVRGMRIRVTDVLDLLAQGLSTEQVLAEMPDLEAEDIAAALQYAARKLNHPILAA

Nucleotide

• Length: 225 bp

>AT28661 NC_019902:3994969-3995193 [Thioalkalivibrio nitratireducens DSM 14787]
ATGAATGACCTTCTGAGGAGAATCACCGTGAACCCCGCACAGTGCGGCGGTCGCCCCTGTGTGCGCGGCATGCGCATTCG
GGTCACCGATGTATTGGACCTGCTGGCGCAGGGCCTCAGCACCGAGCAGGTCCTCGCCGAAATGCCCGATCTCGAGGCGG
AGGACATCGCGGCCGCATTGCAGTACGCCGCACGCAAGCTCAATCACCCGATCCTGGCCGCATGA

Structures

Toxin

• AlphaFold DB: L0E2J6