Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3934794..3935370 Replicon chromosome
Accession NZ_LR134000
Organism Escherichia coli strain NCTC9066

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL014_RS19160 Protein ID WP_001297643.1
Coordinates 3935083..3935370 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EL014_RS19155 Protein ID WP_000063156.1
Coordinates 3934794..3935096 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL014_RS19140 3931390..3933540 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EL014_RS19145 3933590..3933793 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL014_RS19150 3933804..3934760 + 957 WP_001297640.1 GTPase -
EL014_RS19155 3934794..3935096 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EL014_RS19160 3935083..3935370 - 288 WP_001297643.1 BrnT family toxin Toxin
EL014_RS19165 3935569..3936266 - 698 Protein_3710 IS1 family transposase -
EL014_RS19170 3936323..3936418 + 96 Protein_3711 dienelactone hydrolase family protein -
EL014_RS19175 3936564..3937796 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
EL014_RS19180 3937837..3939117 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T286575 WP_001297643.1 NZ_LR134000:c3935370-3935083 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT286575 WP_000063156.1 NZ_LR134000:c3935096-3934794 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References