Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2257318..2257844 Replicon chromosome
Accession NZ_LR134000
Organism Escherichia coli strain NCTC9066

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EL014_RS11035 Protein ID WP_000323025.1
Coordinates 2257557..2257844 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EL014_RS11030 Protein ID WP_000534858.1
Coordinates 2257318..2257557 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL014_RS10995 2252416..2252658 + 243 Protein_2124 DUF977 family protein -
EL014_RS11000 2252655..2253011 + 357 WP_001310834.1 class I SAM-dependent methyltransferase -
EL014_RS22710 2253622..2253909 + 288 Protein_2126 hypothetical protein -
EL014_RS11015 2254678..2255991 + 1314 WP_000589005.1 ISNCY family transposase -
EL014_RS11020 2256453..2256785 - 333 WP_001301033.1 protein FlxA -
EL014_RS11025 2256988..2257293 - 306 WP_001326990.1 hypothetical protein -
EL014_RS11030 2257318..2257557 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EL014_RS11035 2257557..2257844 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EL014_RS11040 2257916..2258071 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EL014_RS11045 2258288..2258539 + 252 WP_000980994.1 hypothetical protein -
EL014_RS11050 2258606..2258884 + 279 WP_012304870.1 hypothetical protein -
EL014_RS11055 2258886..2259935 + 1050 WP_001265198.1 DUF968 domain-containing protein -
EL014_RS11060 2259949..2260701 + 753 WP_001047135.1 antitermination protein -
EL014_RS11065 2260979..2261068 - 90 WP_120795389.1 hypothetical protein -
EL014_RS11070 2261123..2261335 - 213 WP_000087756.1 cold shock-like protein CspF -
EL014_RS11075 2261636..2261851 + 216 WP_000066484.1 cold shock-like protein CspB -
EL014_RS22850 2262215..2262385 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
EL014_RS11080 2262605..2262820 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2237958..2274931 36973


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T286568 WP_000323025.1 NZ_LR134000:2257557-2257844 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT286568 WP_000534858.1 NZ_LR134000:2257318-2257557 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References