Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1595937..1596527 Replicon chromosome
Accession NZ_LR133996
Organism Shimwellia blattae strain NCTC10965

Toxin (Protein)


Gene name pemK Uniprot ID I2B7X3
Locus tag EL017_RS07510 Protein ID WP_002440075.1
Coordinates 1596195..1596527 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I2B7X2
Locus tag EL017_RS07505 Protein ID WP_002440074.1
Coordinates 1595937..1596194 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL017_RS07480 1591122..1592570 - 1449 WP_002440068.1 AMP nucleosidase -
EL017_RS07490 1593705..1594622 + 918 WP_002440070.1 nitrogen assimilation transcriptional regulator NAC -
EL017_RS07495 1594728..1595678 + 951 WP_002440072.1 HTH-type transcriptional regulator Cbl -
EL017_RS07505 1595937..1596194 + 258 WP_002440074.1 hypothetical protein Antitoxin
EL017_RS07510 1596195..1596527 + 333 WP_002440075.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL017_RS07520 1596769..1597554 - 786 WP_002440077.1 DgsA anti-repressor MtfA -
EL017_RS07530 1597911..1598549 + 639 WP_002440078.1 GrpB family protein -
EL017_RS07535 1598695..1598958 + 264 WP_034920043.1 hypothetical protein -
EL017_RS07540 1599131..1600567 + 1437 WP_174270544.1 DNA cytosine methyltransferase -
EL017_RS07545 1600551..1601024 + 474 WP_002440083.1 DNA mismatch endonuclease Vsr -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-85)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11760.52 Da        Isoelectric Point: 10.2965

>T286549 WP_002440075.1 NZ_LR133996:1596195-1596527 [Shimwellia blattae]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFARTAGFAVSLDGAGTKTTGVIRCDQPRTI
DMAARNGKRLERLPDAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9412.93 Da        Isoelectric Point: 4.4750

>AT286549 WP_002440074.1 NZ_LR133996:1595937-1596194 [Shimwellia blattae]
MYTTRLKKVGGSVMLAVPPVVLKTLSLSVDSEVGMTIENGCLIIEPQKRPRYSLEELLAQCEPHAEMSEEDREWIDAPAA
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2B7X3

Antitoxin

Source ID Structure
AlphaFold DB I2B7X2

References