Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5015728..5016353 Replicon chromosome
Accession NZ_LR133964
Organism Klebsiella pneumoniae strain NCTC11359

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EL047_RS24920 Protein ID WP_002882817.1
Coordinates 5015728..5016111 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EL047_RS24925 Protein ID WP_004150355.1
Coordinates 5016111..5016353 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL047_RS24905 5013094..5013996 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EL047_RS24910 5013993..5014628 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EL047_RS24915 5014625..5015554 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EL047_RS24920 5015728..5016111 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EL047_RS24925 5016111..5016353 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EL047_RS24930 5016558..5017475 + 918 WP_080893931.1 alpha/beta hydrolase -
EL047_RS24935 5017489..5018430 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EL047_RS24940 5018475..5018912 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EL047_RS24945 5018909..5019769 - 861 WP_002882807.1 virulence factor BrkB family protein -
EL047_RS24950 5019763..5020362 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T286540 WP_002882817.1 NZ_LR133964:c5016111-5015728 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT286540 WP_004150355.1 NZ_LR133964:c5016353-5016111 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References