Detailed information of TA system TA028654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2119703..2120330
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: L0DVX4
• Locus tag: TVNIR_RS09820
• Protein ID: WP_015258874.1
• Coordinates: 2119703..2119999 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L0DXQ4
• Locus tag: TVNIR_RS09825
• Protein ID: WP_015258875.1
• Coordinates: 2120004..2120330 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS09785	2114714..2115673	+	960	WP_157092252.1	isoaspartyl peptidase/L-asparaginase	-
TVNIR_RS09790	2115717..2116586	-	870	WP_083499428.1	hypothetical protein	-
TVNIR_RS09795	2117044..2117229	+	186	WP_015258868.1	hypothetical protein	-
TVNIR_RS09800	2117222..2117746	+	525	WP_015258869.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
TVNIR_RS09805	2117785..2118351	-	567	WP_015258870.1	hypothetical protein	-
TVNIR_RS19045	2118355..2118654	-	300	WP_015258871.1	hypothetical protein	-
TVNIR_RS09810	2118647..2118853	-	207	WP_043739580.1	hypothetical protein	-
TVNIR_RS09815	2118855..2119586	-	732	WP_083499529.1	hypothetical protein	-
TVNIR_RS09820	2119703..2119999	+	297	WP_015258874.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVNIR_RS09825	2120004..2120330	+	327	WP_015258875.1	putative addiction module antidote protein	Antitoxin
TVNIR_RS09830	2120636..2121445	-	810	WP_043739582.1	hypothetical protein	-
TVNIR_RS09840	2121577..2124167	-	2591	Protein_1983	lytic transglycosylase domain-containing protein	-
TVNIR_RS09845	2124189..2124521	-	333	WP_015258879.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (4-104)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11070.66 Da
• Isoelectric Point: 9.7980

>T28654 WP_015258874.1 NC_019902:2119703-2119999 [Thioalkalivibrio nitratireducens DSM 14787]
MVTIQTTSSFDAWFESLRDRQGKARIEARLRRVEMGHMGDVKAVGKGVQEMRIDCGPGYRIYFTQRGIEIVILLAGGDKD
SQRRDIRAATELARQIGD

Nucleotide

• Length: 297 bp

>T28654 NC_019902:2119703-2119999 [Thioalkalivibrio nitratireducens DSM 14787]
ATGGTCACGATCCAGACAACCAGCTCGTTCGATGCGTGGTTCGAGTCCCTGCGGGACCGGCAGGGCAAGGCCCGGATCGA
AGCCCGGCTGCGGCGCGTAGAGATGGGTCACATGGGCGACGTGAAGGCGGTCGGCAAGGGCGTGCAGGAGATGCGGATCG
ACTGCGGACCCGGATACCGGATCTACTTCACCCAGCGGGGCATCGAGATCGTGATCTTATTGGCTGGTGGCGACAAGGAT
TCCCAGCGACGGGACATCCGCGCAGCCACCGAACTGGCCCGGCAGATTGGAGACTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11967.67 Da
• Isoelectric Point: 6.2266

>AT28654 WP_015258875.1 NC_019902:2120004-2120330 [Thioalkalivibrio nitratireducens DSM 14787]
MTNVTLKRWDVVDHLRTDEEMALYLDACFAEDPGDGSLIRVALGDIARARGMTQLARDTGMAREGLYKALSAEGNPEFAT
IMKVVRALRLRLHAHSASENREPSILAQ

Nucleotide

• Length: 327 bp

>AT28654 NC_019902:2120004-2120330 [Thioalkalivibrio nitratireducens DSM 14787]
ATGACCAACGTGACGCTGAAGCGCTGGGACGTGGTGGACCATCTCCGCACCGACGAAGAGATGGCGCTCTATCTCGACGC
CTGCTTCGCTGAAGATCCAGGGGACGGGAGCCTGATCCGGGTCGCTCTCGGAGACATTGCCAGAGCACGCGGCATGACCC
AACTGGCCCGGGATACCGGCATGGCTCGCGAGGGTCTGTACAAGGCGCTGTCCGCTGAAGGGAATCCGGAGTTCGCGACG
ATCATGAAGGTCGTGCGAGCCCTCCGGCTTCGGCTGCATGCCCACAGCGCGAGCGAAAACCGCGAGCCGAGCATCCTGGC
GCAGTAG

Structures

Toxin

• AlphaFold DB: L0DVX4

Antitoxin

• AlphaFold DB: L0DXQ4