Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4624022..4624519 Replicon chromosome
Accession NZ_LR130564
Organism Escherichia coli strain MS14387 isolate MS14387

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FVE81_RS22645 Protein ID WP_001173343.1
Coordinates 4624022..4624195 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FVE81_RS22650 Protein ID WP_000398619.1
Coordinates 4624223..4624519 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE81_RS22635 4620693..4622150 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
FVE81_RS22640 4622387..4623904 + 1518 WP_000003806.1 lysine--tRNA ligase -
FVE81_RS22645 4624022..4624195 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FVE81_RS22650 4624223..4624519 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FVE81_RS22655 4624748..4625020 - 273 WP_000405647.1 N-acetyltransferase -
FVE81_RS22660 4625032..4625262 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
FVE81_RS22665 4625443..4627074 + 1632 WP_001216477.1 sensor histidine kinase -
FVE81_RS22670 4627071..4627790 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4615169..4624519 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T286498 WP_001173343.1 NZ_LR130564:c4624195-4624022 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT286498 WP_000398619.1 NZ_LR130564:c4624519-4624223 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References