286469

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4265423..4265999 Replicon chromosome
Accession NZ_LR130562
Organism Escherichia coli strain MS14384 isolate MS14384

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag EW037_RS20625 Protein ID WP_001131965.1
Coordinates 4265712..4265999 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag EW037_RS20620 Protein ID WP_019842999.1
Coordinates 4265423..4265725 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW037_RS20600 4261117..4261629 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
EW037_RS20605 4262019..4264169 + 2151 WP_001439331.1 pyruvate/proton symporter BtsT -
EW037_RS20610 4264219..4264422 + 204 WP_000467859.1 YbdD/YjiX family protein -
EW037_RS20615 4264433..4265389 + 957 WP_001295525.1 GTPase -
EW037_RS20620 4265423..4265725 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
EW037_RS20625 4265712..4265999 - 288 WP_001131965.1 BrnT family toxin Toxin
EW037_RS20630 4266196..4267362 + 1167 WP_000800825.1 restriction endonuclease -
EW037_RS20635 4267426..4269045 + 1620 WP_001029743.1 type I restriction-modification system subunit M -
EW037_RS20640 4269035..4270459 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T286469 WP_001131965.1 NZ_LR130562:c4265999-4265712 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT286469 WP_019842999.1 NZ_LR130562:c4265725-4265423 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References