Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2013..2620 Replicon plasmid 4
Accession NZ_LR130558
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag EW040_RS26635 Protein ID WP_000673985.1
Coordinates 2013..2399 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag EW040_RS26640 Protein ID WP_000493532.1
Coordinates 2396..2620 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS26625 1..858 + 858 WP_000130985.1 incFII family plasmid replication initiator RepA -
EW040_RS26635 2013..2399 - 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EW040_RS26640 2396..2620 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EW040_RS26645 2800..3081 - 282 WP_001290347.1 membrane protein -
EW040_RS26650 3243..3527 - 285 WP_000930236.1 hypothetical protein -
EW040_RS26655 3563..3865 - 303 WP_001096541.1 hypothetical protein -
EW040_RS26660 3955..4614 - 660 WP_016235966.1 AAA family ATPase -
EW040_RS26665 5166..5489 + 324 WP_016235965.1 hypothetical protein -
EW040_RS26670 5843..6187 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
EW040_RS26675 6174..6524 + 351 WP_000127482.1 addiction module antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..52792 52792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T286449 WP_000673985.1 NZ_LR130558:c2399-2013 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT286449 WP_000493532.1 NZ_LR130558:c2620-2396 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References