Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 104691..105216 Replicon plasmid 2
Accession NZ_LR130556
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EW040_RS25795 Protein ID WP_001159868.1
Coordinates 104691..104996 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EW040_RS25800 Protein ID WP_000813634.1
Coordinates 104998..105216 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS25780 100601..101767 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EW040_RS25785 102355..103110 - 756 WP_024946706.1 replication initiation protein RepE -
EW040_RS25790 103884..104690 - 807 WP_000016982.1 site-specific integrase -
EW040_RS25795 104691..104996 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EW040_RS25800 104998..105216 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EW040_RS25805 105783..106295 + 513 WP_000151784.1 hypothetical protein -
EW040_RS25810 106329..107462 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
EW040_RS25815 107629..108402 - 774 WP_000905949.1 hypothetical protein -
EW040_RS25820 108415..108915 - 501 WP_000528931.1 hypothetical protein -
EW040_RS25825 109180..109410 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EW040_RS25830 109407..109823 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / mph(A) / sul1 / qacE / aadA5 / sitABCD iucA / iucB / iucC / iucD / iutA 1..136397 136397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T286445 WP_001159868.1 NZ_LR130556:c104996-104691 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT286445 WP_000813634.1 NZ_LR130556:c105216-104998 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References