Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4529248..4529745 Replicon chromosome
Accession NZ_LR130555
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EW040_RS22250 Protein ID WP_001173343.1
Coordinates 4529248..4529421 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EW040_RS22255 Protein ID WP_000398619.1
Coordinates 4529449..4529745 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS22240 4525918..4527375 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
EW040_RS22245 4527612..4529129 + 1518 WP_022646456.1 lysine--tRNA ligase -
EW040_RS22250 4529248..4529421 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EW040_RS22255 4529449..4529745 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EW040_RS22260 4529974..4530246 - 273 WP_000405647.1 N-acetyltransferase -
EW040_RS22265 4530258..4530488 - 231 WP_001683478.1 (4Fe-4S)-binding protein -
EW040_RS22270 4530669..4532300 + 1632 WP_022646455.1 sensor histidine kinase -
EW040_RS22275 4532297..4533016 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T286436 WP_001173343.1 NZ_LR130555:c4529421-4529248 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT286436 WP_000398619.1 NZ_LR130555:c4529745-4529449 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References