Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4285884..4286460 Replicon chromosome
Accession NZ_LR130555
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EW040_RS20925 Protein ID WP_001297643.1
Coordinates 4286173..4286460 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EW040_RS20920 Protein ID WP_000063156.1
Coordinates 4285884..4286186 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS20905 4282480..4284630 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EW040_RS20910 4284680..4284883 + 204 WP_000467859.1 YbdD/YjiX family protein -
EW040_RS20915 4284894..4285850 + 957 WP_001297640.1 GTPase -
EW040_RS20920 4285884..4286186 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EW040_RS20925 4286173..4286460 - 288 WP_001297643.1 BrnT family toxin Toxin
EW040_RS20930 4286659..4287356 - 698 Protein_4043 IS1 family transposase -
EW040_RS20935 4287413..4287508 + 96 Protein_4044 dienelactone hydrolase family protein -
EW040_RS20940 4287654..4288886 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EW040_RS20945 4288927..4290207 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T286434 WP_001297643.1 NZ_LR130555:c4286460-4286173 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT286434 WP_000063156.1 NZ_LR130555:c4286186-4285884 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References