Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3049794..3050578 Replicon chromosome
Accession NZ_LR130555
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag EW040_RS14895 Protein ID WP_000613626.1
Coordinates 3050084..3050578 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag EW040_RS14890 Protein ID WP_024946541.1
Coordinates 3049794..3050087 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS14875 3044955..3045914 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
EW040_RS14885 3046487..3049660 + 3174 WP_022645526.1 ribonuclease E -
EW040_RS14890 3049794..3050087 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
EW040_RS14895 3050084..3050578 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
EW040_RS14900 3050673..3051626 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
EW040_RS14905 3051638..3053281 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
EW040_RS14910 3053347..3054288 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
EW040_RS14915 3054288..3055385 - 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T286428 WP_000613626.1 NZ_LR130555:3050084-3050578 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT286428 WP_024946541.1 NZ_LR130555:3049794-3050087 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References