Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4345000..4345497 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EW035_RS21050 Protein ID WP_001173343.1
Coordinates 4345000..4345173 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EW035_RS21055 Protein ID WP_000398619.1
Coordinates 4345201..4345497 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS21040 4341670..4343127 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
EW035_RS21045 4343364..4344881 + 1518 WP_023155344.1 lysine--tRNA ligase -
EW035_RS21050 4345000..4345173 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EW035_RS21055 4345201..4345497 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EW035_RS21060 4345724..4345996 - 273 WP_000405647.1 N-acetyltransferase -
EW035_RS21065 4346008..4346238 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
EW035_RS21070 4346419..4348050 + 1632 WP_001216460.1 sensor histidine kinase -
EW035_RS21075 4348047..4348766 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T286410 WP_001173343.1 NZ_LR130552:c4345173-4345000 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT286410 WP_000398619.1 NZ_LR130552:c4345497-4345201 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References