Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4109464..4110040 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag EW035_RS19845 Protein ID WP_001350372.1
Coordinates 4109753..4110040 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag EW035_RS19840 Protein ID WP_001402821.1
Coordinates 4109464..4109766 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS19820 4105155..4105667 + 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
EW035_RS19825 4106060..4108210 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
EW035_RS19830 4108260..4108463 + 204 WP_000467859.1 YbdD/YjiX family protein -
EW035_RS19835 4108474..4109430 + 957 WP_001295525.1 GTPase -
EW035_RS19840 4109464..4109766 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
EW035_RS19845 4109753..4110040 - 288 WP_001350372.1 BrnT family toxin Toxin
EW035_RS19850 4110237..4111403 + 1167 WP_001672354.1 restriction endonuclease -
EW035_RS19855 4111467..4113086 + 1620 WP_001029742.1 type I restriction-modification system subunit M -
EW035_RS19860 4113076..4114458 + 1383 WP_023155808.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T286408 WP_001350372.1 NZ_LR130552:c4110040-4109753 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT286408 WP_001402821.1 NZ_LR130552:c4109766-4109464 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References