Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1362297..1362922 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EW035_RS06555 Protein ID WP_000911329.1
Coordinates 1362524..1362922 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EW035_RS06550 Protein ID WP_000450524.1
Coordinates 1362297..1362524 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS06525 1358100..1358570 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
EW035_RS06530 1358570..1359142 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EW035_RS06535 1359288..1360166 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EW035_RS06540 1360183..1361217 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EW035_RS06545 1361430..1362143 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EW035_RS06550 1362297..1362524 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EW035_RS06555 1362524..1362922 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
EW035_RS06560 1363069..1363932 + 864 WP_001267498.1 neutral zinc metallopeptidase -
EW035_RS06565 1363947..1365962 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
EW035_RS06570 1366036..1366734 + 699 WP_000679823.1 esterase -
EW035_RS06575 1366844..1367044 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T286398 WP_000911329.1 NZ_LR130552:1362524-1362922 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT286398 WP_000450524.1 NZ_LR130552:1362297-1362524 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References