Detailed information of TA system TA028639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 41461..42002
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0DQL5
• Locus tag: TVNIR_RS00260
• Protein ID: WP_015256929.1
• Coordinates: 41679..42002 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TVNIR_RS18375
• Protein ID: WP_083499508.1
• Coordinates: 41461..41682 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS00240	37007..38893	-	1887	WP_015256924.1	hypothetical protein	-
TVNIR_RS00245	38970..39404	-	435	WP_015256925.1	pilin	-
TVNIR_RS00250	40144..40704	+	561	WP_211263133.1	Uma2 family endonuclease	-
TVNIR_RS00255	40780..41364	+	585	WP_043738944.1	Uma2 family endonuclease	-
TVNIR_RS18375	41461..41682	+	222	WP_083499508.1	antitoxin MazE family protein	Antitoxin
TVNIR_RS00260	41679..42002	+	324	WP_015256929.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TVNIR_RS00265	42257..43975	-	1719	WP_015256930.1	NADH/Ubiquinone/plastoquinone (complex I)	-
TVNIR_RS00270	44454..45461	+	1008	WP_015256931.1	NAD-dependent epimerase	-
TVNIR_RS00275	45883..46371	+	489	WP_015256933.1	transcriptional activator RfaH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-71)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11445.37 Da
• Isoelectric Point: 10.2913

>T28639 WP_015256929.1 NC_019902:41679-42002 [Thioalkalivibrio nitratireducens DSM 14787]
MKRGDLVTVAMSGDFGKPRPALVIQSDHFDATATVTVLLVSSTLVDAPLIRLAVEPSAENGLRKPSQVMIDKATTVRRDR
VGAPIGRLEAETMVVVNRSLALFLGFA

Nucleotide

• Length: 324 bp

>T28639 NC_019902:41679-42002 [Thioalkalivibrio nitratireducens DSM 14787]
ATGAAGCGCGGAGATCTGGTCACGGTGGCGATGTCGGGTGATTTCGGCAAGCCCCGGCCGGCGCTGGTGATTCAGTCCGA
CCACTTCGACGCTACGGCGACGGTGACGGTGCTTCTGGTTTCTTCGACGCTCGTCGACGCTCCGCTGATCCGGTTGGCGG
TCGAGCCGAGCGCGGAGAATGGCCTGCGCAAGCCATCGCAGGTGATGATCGACAAGGCGACGACGGTGCGGCGGGATCGG
GTGGGCGCACCCATCGGTCGACTGGAAGCCGAGACCATGGTTGTGGTGAACCGCAGCCTCGCGCTGTTTCTCGGGTTTGC
GTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8242.39 Da
• Isoelectric Point: 4.9802

>AT28639 WP_083499508.1 NC_019902:41461-41682 [Thioalkalivibrio nitratireducens DSM 14787]
MPMSVNERVQKRRALLRASGLRPLQIWVPDTRQPGFVEECRRQSMVVARTDLGDPDVDAFMQGALADADDWTA

Nucleotide

• Length: 222 bp

>AT28639 NC_019902:41461-41682 [Thioalkalivibrio nitratireducens DSM 14787]
ATGCCCATGTCAGTGAATGAACGTGTACAAAAGCGGCGCGCCCTGCTGCGAGCGTCCGGTTTACGGCCTCTGCAGATTTG
GGTGCCGGATACGCGGCAGCCGGGCTTCGTGGAGGAATGCCGGCGTCAATCGATGGTCGTCGCCCGGACGGATCTCGGCG
ATCCCGATGTGGACGCCTTCATGCAGGGCGCCCTGGCGGATGCCGACGACTGGACCGCATGA

Structures

Toxin

• AlphaFold DB: L0DQL5