Detailed information of TA system TA028638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-PrlF
• Location: 26082..26646
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0DQK6
• Locus tag: TVNIR_RS00190
• Protein ID: WP_006746522.1
• Coordinates: 26302..26646 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L0DRW8
• Locus tag: TVNIR_RS00185
• Protein ID: WP_006746523.1
• Coordinates: 26082..26312 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS00140	21544..21888	-	345	WP_211263161.1	toxin-antitoxin system HicB family antitoxin	-
TVNIR_RS00145	21894..22148	-	255	WP_015256908.1	type II toxin-antitoxin system HicA family toxin	-
TVNIR_RS00150	22363..22785	-	423	WP_015256909.1	DUF86 domain-containing protein	-
TVNIR_RS00155	22785..23192	-	408	WP_043738930.1	nucleotidyltransferase domain-containing protein	-
TVNIR_RS00160	23862..24086	+	225	WP_015256911.1	antitoxin VapB	-
TVNIR_RS00165	24261..24527	+	267	WP_083499307.1	type II toxin-antitoxin system CcdA family antitoxin	-
TVNIR_RS00170	24527..24841	+	315	WP_015256913.1	CcdB family protein	-
TVNIR_RS00175	24911..25306	-	396	WP_015256914.1	type II toxin-antitoxin system VapC family toxin	-
TVNIR_RS00180	25311..25550	-	240	WP_006746524.1	hypothetical protein	-
TVNIR_RS00185	26082..26312	+	231	WP_006746523.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
TVNIR_RS00190	26302..26646	+	345	WP_006746522.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TVNIR_RS00195	26664..27512	-	849	WP_015256915.1	transposase	-
TVNIR_RS00200	27574..28524	-	951	WP_083499308.1	IS4 family transposase	-
TVNIR_RS00205	28881..29228	+	348	WP_043738935.1	hypothetical protein	-
TVNIR_RS00210	29287..29954	+	668	Protein_42	DNA methylase	-
TVNIR_RS00215	29970..31403	+	1434	WP_015256918.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

• SpoVT-AbrB domain (5-31)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12168.08 Da
• Isoelectric Point: 10.7923

>T28638 WP_006746522.1 NC_019902:26302-26646 [Thioalkalivibrio nitratireducens DSM 14787]
VTYDRFTVVRVPFPFTDRSAVKNRPALVLSDAAAFSATAGHSVMAMITSKGNAPWPLDCSISELDAAGLPAPSVVRFKLF
TLDHRLIRSTLGQLAEADRATVRSALKRLLGGAA

Nucleotide

• Length: 345 bp

>T28638 NC_019902:26302-26646 [Thioalkalivibrio nitratireducens DSM 14787]
GTGACCTATGACCGCTTCACGGTCGTGCGCGTGCCGTTTCCGTTCACCGATCGCAGCGCTGTCAAGAACCGCCCGGCCCT
GGTGCTTTCGGATGCCGCGGCCTTTAGCGCGACCGCTGGGCACTCGGTGATGGCGATGATTACTTCGAAGGGCAACGCAC
CCTGGCCACTCGATTGCTCAATCAGCGAACTTGACGCCGCCGGCCTGCCGGCGCCGTCCGTGGTGCGCTTCAAGCTGTTT
ACCCTCGATCACCGCCTGATTCGGAGCACCCTGGGCCAACTCGCCGAGGCCGACCGTGCAACCGTGCGCAGCGCATTGAA
GCGCTTGCTGGGCGGCGCCGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8350.46 Da
• Isoelectric Point: 4.9890

>AT28638 WP_006746523.1 NC_019902:26082-26312 [Thioalkalivibrio nitratireducens DSM 14787]
MPAIARITAKGQTTIPKQVRAALHVAPGDLIAWEIHPDGTASVRRVQPLDVDYLRALEATLSEWSSPADEEAYRDL

Nucleotide

• Length: 231 bp

>AT28638 NC_019902:26082-26312 [Thioalkalivibrio nitratireducens DSM 14787]
ATGCCCGCCATCGCGAGAATTACCGCCAAAGGCCAGACGACCATTCCGAAGCAGGTGCGCGCTGCCTTGCATGTCGCACC
CGGCGACCTCATCGCATGGGAGATCCATCCTGATGGGACTGCGTCGGTGCGGCGCGTGCAGCCTCTGGACGTGGACTACC
TGCGCGCCCTCGAAGCAACCCTGTCCGAGTGGTCCAGCCCGGCCGATGAGGAAGCTTACCGTGACCTATGA

Structures

Toxin

• AlphaFold DB: L0DQK6

Antitoxin

• AlphaFold DB: L0DRW8