Detailed information of TA system TA028636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3057553..3058159
• Replicon: chromosome
• Accession: NC_019897
• Organism: Thermobacillus composti KWC4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0EGS2
• Locus tag: THECO_RS14225
• Protein ID: WP_015255736.1
• Coordinates: 3057553..3057903 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THECO_RS14230
• Protein ID: WP_198285810.1
• Coordinates: 3057908..3058159 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THECO_RS14200	3052994..3053938	-	945	WP_015255731.1	hypothetical protein	-
THECO_RS14205	3053932..3055263	-	1332	WP_015255732.1	Flp pilus assembly complex ATPase component TadA	-
THECO_RS14210	3055263..3056099	-	837	WP_015255733.1	hypothetical protein	-
THECO_RS14215	3056096..3056836	-	741	WP_015255734.1	SAF domain-containing protein	-
THECO_RS14220	3057026..3057289	-	264	WP_086010533.1	ribbon-helix-helix protein, CopG family	-
THECO_RS14225	3057553..3057903	-	351	WP_015255736.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THECO_RS14230	3057908..3058159	-	252	WP_198285810.1	ribbon-helix-helix protein, CopG family	Antitoxin
THECO_RS14235	3058308..3059486	-	1179	WP_015255738.1	alanine racemase	-
THECO_RS14240	3059738..3060991	-	1254	WP_015255739.1	DUF4367 domain-containing protein	-
THECO_RS14245	3061499..3061717	-	219	WP_015255740.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12767.73 Da
• Isoelectric Point: 5.6377

>T28636 WP_015255736.1 NC_019897:c3057903-3057553 [Thermobacillus composti KWC4]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVELSAEAHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDDETMKKVDESLSISVGLIDF

Nucleotide

• Length: 351 bp

>T28636 NC_019897:c3057903-3057553 [Thermobacillus composti KWC4]
TTGATCGTAAAACGCGGGGATGTGTTTTTCGCAGATTTGTCGCCGGTGGTCGGATCAGAGCAGGGCGGCGTACGCCCCGT
GCTGATCATCCAGAACGACATCGGCAACCGGTTCAGCCCGACGGTGATTGTCGCGGCGATTACGGCGAAGATTCAGAAAG
CGAAGCTGCCCACCCATGTCGAGCTCAGCGCGGAAGCGCACGGCTTCGACCGGGATTCGGTCGTGCTGCTCGAGCAGATC
CGCACGATCGACAAGCAGCGTCTTACGGACAAAATCACGCATCTGGACGACGAGACGATGAAGAAAGTCGACGAATCGCT
CTCCATCAGTGTCGGTCTGATCGATTTCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9554.92 Da
• Isoelectric Point: 5.8506

>AT28636 WP_198285810.1 NC_019897:c3058159-3057908 [Thermobacillus composti KWC4]
MISLPDQLLREVDGIVAREKSNRSEFIRQAMKLYLTERKKRQIRDSMQRGYLEMANINLHMAAEAFQAEDDADDTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT28636 NC_019897:c3058159-3057908 [Thermobacillus composti KWC4]
ATGATCAGTCTGCCGGATCAGTTGCTTCGGGAAGTGGACGGGATCGTGGCGCGAGAGAAGTCGAACCGCAGCGAATTCAT
CCGCCAGGCGATGAAGCTCTATCTTACCGAACGGAAGAAGCGTCAAATCCGTGATTCCATGCAGCGGGGATATCTCGAGA
TGGCGAACATCAACCTGCACATGGCCGCTGAAGCATTCCAGGCGGAGGATGATGCCGACGACACGCTCGGCCGTCTCGTA
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: L0EGS2