Detailed information of TA system TA028635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 143726..144289
• Replicon: chromosome
• Accession: NC_019897
• Organism: Thermobacillus composti KWC4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0E9M1
• Locus tag: THECO_RS00620
• Protein ID: WP_015253143.1
• Coordinates: 143726..144049 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THECO_RS00625
• Protein ID: WP_157373487.1
• Coordinates: 144050..144289 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THECO_RS00595	138919..139221	-	303	WP_015253138.1	helix-turn-helix domain-containing protein	-
THECO_RS00600	139257..139826	-	570	WP_015253139.1	hypothetical protein	-
THECO_RS00605	140148..140828	+	681	WP_157373486.1	hypothetical protein	-
THECO_RS00610	141483..142874	+	1392	WP_015253141.1	group II intron reverse transcriptase/maturase	-
THECO_RS21500	143010..143222	-	213	WP_015253142.1	hypothetical protein	-
THECO_RS00620	143726..144049	-	324	WP_015253143.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THECO_RS00625	144050..144289	-	240	WP_157373487.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
THECO_RS00630	144436..144915	-	480	WP_015253145.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
THECO_RS00635	144922..145389	-	468	WP_015253146.1	MerR family transcriptional regulator	-
THECO_RS00640	145552..146403	+	852	WP_015253147.1	aldo/keto reductase	-
THECO_RS00645	146499..147890	-	1392	WP_015253148.1	group II intron reverse transcriptase/maturase	-
THECO_RS00650	148357..148524	-	168	WP_015253149.1	CxxH/CxxC protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-106)

Antitoxin

• SpoVT-AbrB domain (6-51)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11911.86 Da
• Isoelectric Point: 7.8896

>T28635 WP_015253143.1 NC_019897:c144049-143726 [Thermobacillus composti KWC4]
MVADRGDLVWINFNPQAGHEQAGRRAAIVLSPKSFNKVTGFVSVCPITHTIRGWGYEVLLPEGLVFKGVVLTDQIKNLDW
KARGIEIVGKAPEEVVRECLDKIHTFL

Nucleotide

• Length: 324 bp

>T28635 NC_019897:c144049-143726 [Thermobacillus composti KWC4]
ATGGTTGCGGACCGGGGAGATCTGGTTTGGATCAACTTCAATCCTCAAGCGGGCCACGAACAAGCCGGCAGAAGAGCTGC
GATCGTGCTTTCTCCAAAATCATTCAACAAAGTGACGGGATTTGTTTCGGTATGTCCGATAACGCATACGATCCGGGGAT
GGGGATATGAAGTGCTCCTGCCCGAAGGATTGGTGTTCAAGGGTGTTGTTTTGACGGATCAGATCAAGAATTTGGACTGG
AAAGCAAGAGGAATTGAAATTGTCGGTAAAGCCCCCGAAGAAGTCGTTCGGGAATGCCTTGATAAAATTCATACTTTCCT
GTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8804.13 Da
• Isoelectric Point: 5.0994

>AT28635 WP_157373487.1 NC_019897:c144289-144050 [Thermobacillus composti KWC4]
MSTTVQKWGNSLGIRIPSHIAEKIAITQGSEVELVVSEDHTLIVIPIKKKPTLEELLAQCKPENRHDEIDFGTEGKELL

Nucleotide

• Length: 240 bp

>AT28635 NC_019897:c144289-144050 [Thermobacillus composti KWC4]
ATGTCGACGACTGTCCAGAAGTGGGGCAACAGTCTGGGGATTCGGATTCCCAGCCACATCGCGGAGAAGATTGCGATTAC
CCAAGGTTCGGAAGTCGAACTGGTTGTCAGTGAAGATCATACGCTTATTGTCATCCCCATAAAGAAAAAGCCGACGCTGG
AAGAATTGCTGGCTCAATGCAAACCGGAAAATCGGCACGATGAGATCGATTTCGGCACAGAAGGGAAGGAACTGCTGTAG

Structures

Toxin

• AlphaFold DB: L0E9M1