Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 360347..361147 Replicon chromosome
Accession NZ_LR130545
Organism Escherichia coli strain B36 isolate B36

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag EW036_RS01735 Protein ID WP_000342452.1
Coordinates 360620..361147 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag EW036_RS01730 Protein ID WP_001277107.1
Coordinates 360347..360613 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW036_RS01710 356006..356674 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
EW036_RS01715 356667..357725 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
EW036_RS01720 357970..358824 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EW036_RS01725 359095..360198 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EW036_RS01730 360347..360613 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
EW036_RS01735 360620..361147 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
EW036_RS01740 361144..361527 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
EW036_RS01745 361950..363059 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EW036_RS01750 363107..364033 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EW036_RS01755 364030..365307 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
EW036_RS01760 365304..366071 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T286347 WP_000342452.1 NZ_LR130545:360620-361147 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT286347 WP_001277107.1 NZ_LR130545:360347-360613 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References