Detailed information of TA system TA028634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3521578..3522214
• Replicon: chromosome
• Accession: NC_019896
• Organism: Bacillus subtilis subsp. subtilis str. BSP1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A7A1_RS18160
• Protein ID: WP_003156187.1
• Coordinates: 3521578..3521928 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: A7A1_RS18165
• Protein ID: WP_003225183.1
• Coordinates: 3521933..3522214 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A1_RS18120	3516622..3517221	-	600	WP_003246608.1	phosphoserine phosphatase RsbX	-
A7A1_RS18125	3517221..3518009	-	789	WP_044052630.1	RNA polymerase sigma factor SigB	-
A7A1_RS18130	3517975..3518457	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
A7A1_RS18135	3518454..3518783	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
A7A1_RS18140	3518845..3519852	-	1008	WP_015252763.1	phosphoserine phosphatase RsbU	-
A7A1_RS18145	3519864..3520264	-	401	Protein_3527	serine/threonine-protein kinase RsbT	-
A7A1_RS18150	3520268..3520633	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
A7A1_RS18155	3520638..3521462	-	825	WP_015252765.1	RsbT co-antagonist protein RsbRA	-
A7A1_RS18160	3521578..3521928	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A7A1_RS18165	3521933..3522214	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A7A1_RS18170	3522330..3523499	-	1170	WP_015252766.1	alanine racemase	-
A7A1_RS18175	3523614..3524630	-	1017	WP_080019556.1	outer membrane lipoprotein carrier protein LolA	-
A7A1_RS18180	3524796..3525161	-	366	WP_015252768.1	holo-ACP synthase	-
A7A1_RS18185	3525256..3525855	+	600	WP_015252769.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T28634 WP_003156187.1 NC_019896:c3521928-3521578 [Bacillus subtilis subsp. subtilis str. BSP1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T28634 NC_019896:c3521928-3521578 [Bacillus subtilis subsp. subtilis str. BSP1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT28634 WP_003225183.1 NC_019896:c3522214-3521933 [Bacillus subtilis subsp. subtilis str. BSP1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT28634 NC_019896:c3522214-3521933 [Bacillus subtilis subsp. subtilis str. BSP1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32