Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 281456..282087 Replicon chromosome
Accession NZ_LR130544
Organism Klebsiella variicola strain 03-311-0071 isolate 03-311-0071

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H4S6Z2
Locus tag EW041_RS01290 Protein ID WP_008806943.1
Coordinates 281456..281731 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag EW041_RS01295 Protein ID WP_004181489.1
Coordinates 281728..282087 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW041_RS01270 276692..277027 + 336 WP_002921203.1 universal stress protein UspB -
EW041_RS01275 277087..278583 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
EW041_RS01280 278813..280006 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
EW041_RS01285 280046..281059 - 1014 WP_012540371.1 magnesium transporter -
EW041_RS01290 281456..281731 + 276 WP_008806943.1 type II toxin-antitoxin system HicA family toxin Toxin
EW041_RS01295 281728..282087 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW041_RS01300 282115..282516 - 402 WP_048268290.1 nickel-responsive transcriptional regulator NikR -
EW041_RS01305 282504..283295 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
EW041_RS01310 283292..284056 - 765 WP_048272087.1 nickel import ATP-binding protein NikD -
EW041_RS01315 284056..284889 - 834 WP_008806947.1 nickel ABC transporter permease subunit NikC -
EW041_RS01320 284886..285830 - 945 WP_129765552.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10295.87 Da        Isoelectric Point: 10.3900

>T286333 WP_008806943.1 NZ_LR130544:281456-281731 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVRP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT286333 WP_004181489.1 NZ_LR130544:281728-282087 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4S6Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References