Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5290946..5291571 Replicon chromosome
Accession NZ_LR130543
Organism Klebsiella variicola strain 04153260899A isolate 04153260899A

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag EW039_RS26050 Protein ID WP_008807903.1
Coordinates 5290946..5291329 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EW039_RS26055 Protein ID WP_004150355.1
Coordinates 5291329..5291571 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW039_RS26035 5288313..5289215 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EW039_RS26040 5289212..5289847 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
EW039_RS26045 5289844..5290773 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EW039_RS26050 5290946..5291329 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
EW039_RS26055 5291329..5291571 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EW039_RS26060 5291776..5292693 + 918 WP_032756518.1 alpha/beta hydrolase -
EW039_RS26065 5292708..5293649 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
EW039_RS26070 5293694..5294131 - 438 WP_008807906.1 D-tyrosyl-tRNA(Tyr) deacylase -
EW039_RS26075 5294128..5294988 - 861 WP_008807907.1 virulence factor BrkB family protein -
EW039_RS26080 5294982..5295581 - 600 WP_064142974.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T286332 WP_008807903.1 NZ_LR130543:c5291329-5290946 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT286332 WP_004150355.1 NZ_LR130543:c5291571-5291329 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References