Detailed information of TA system TA286331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4970276..4971957
• Replicon: chromosome
• Accession: NZ_LR130543
• Organism: Klebsiella variicola strain 04153260899A isolate 04153260899A

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: EW039_RS24505
• Protein ID: WP_032734038.1
• Coordinates: 4970276..4971193 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: EW039_RS24510
• Protein ID: WP_117107357.1
• Coordinates: 4971199..4971957 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW039_RS24485	4965582..4967102	+	1521	WP_129678786.1	sugar ABC transporter ATP-binding protein	-
EW039_RS24490	4967099..4968097	+	999	WP_023296904.1	ribose ABC transporter permease	-
EW039_RS24495	4968128..4969069	+	942	WP_008807254.1	ABC transporter substrate-binding protein	-
EW039_RS24500	4969144..4970151	+	1008	WP_129678787.1	carbohydrate kinase family protein	-
EW039_RS24505	4970276..4971193	-	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
EW039_RS24510	4971199..4971957	-	759	WP_117107357.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
EW039_RS24515	4972042..4972770	-	729	WP_008807258.1	response regulator transcription factor	-
EW039_RS24520	4973043..4975190	+	2148	WP_077598880.1	formate dehydrogenase subunit alpha	-
EW039_RS24525	4975896..4976582	+	687	WP_008807260.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T286331 WP_032734038.1 NZ_LR130543:c4971193-4970276 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28683.04 Da
• Isoelectric Point: 10.2152

>AT286331 WP_117107357.1 NZ_LR130543:c4971957-4971199 [Klebsiella variicola]
MSSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2