Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1746807..1747397 Replicon chromosome
Accession NZ_LR130543
Organism Klebsiella variicola strain 04153260899A isolate 04153260899A

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag EW039_RS08575 Protein ID WP_008804165.1
Coordinates 1747065..1747397 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag EW039_RS08570 Protein ID WP_012541132.1
Coordinates 1746807..1747064 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW039_RS08545 1742414..1742989 + 576 WP_129678569.1 hypothetical protein -
EW039_RS08550 1743167..1744003 + 837 WP_008804155.1 alpha/beta hydrolase -
EW039_RS08555 1744210..1745181 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
EW039_RS08560 1745178..1746278 - 1101 WP_086631305.1 phosphotransferase -
EW039_RS08570 1746807..1747064 + 258 WP_012541132.1 antitoxin Antitoxin
EW039_RS08575 1747065..1747397 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EW039_RS08580 1748157..1749971 + 1815 WP_064171031.1 hypothetical protein -
EW039_RS08585 1750022..1750948 + 927 WP_110215056.1 hypothetical protein -
EW039_RS08590 1750941..1751399 + 459 WP_086631304.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T286322 WP_008804165.1 NZ_LR130543:1747065-1747397 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT286322 WP_012541132.1 NZ_LR130543:1746807-1747064 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References