Detailed information of TA system TA028632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1684756..1685290
• Replicon: plasmid pRmeGR4d
• Accession: NC_019849
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C770_RS49720
• Protein ID: WP_015243550.1
• Coordinates: 1684756..1685052 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: C770_RS49725
• Protein ID: WP_003527850.1
• Coordinates: 1685042..1685290 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS49710	1680689..1682023	-	1335	WP_015242562.1	ISNCY-like element ISRm17 family transposase	-
C770_RS49715	1682227..1683486	-	1260	WP_015241829.1	group II intron reverse transcriptase/maturase	-
C770_RS52760	1684415..1684675	+	261	Protein_1512	helix-turn-helix domain containing protein	-
C770_RS49720	1684756..1685052	-	297	WP_015243550.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C770_RS49725	1685042..1685290	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
C770_RS53550	1685396..1685551	-	156	WP_127642757.1	hypothetical protein	-
C770_RS49735	1685907..1686032	+	126	Protein_1516	IS481 family transposase	-
C770_RS49740	1686116..1686910	-	795	WP_015243551.1	methyltransferase domain-containing protein	-
C770_RS49745	1686914..1687714	-	801	WP_010974981.1	ABC transporter ATP-binding protein	-
C770_RS49750	1687711..1688754	-	1044	WP_015243552.1	iron ABC transporter permease	-
C770_RS49755	1688751..1689818	-	1068	WP_010974979.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1701381	1701381
	-	inside	IScluster/Tn	-	-	1680689..1686032	5343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11442.14 Da
• Isoelectric Point: 10.7543

>T28632 WP_015243550.1 NC_019849:c1685052-1684756 [Sinorhizobium meliloti GR4]
MATERPFRLAPAAKADLRKIWRYKARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T28632 NC_019849:c1685052-1684756 [Sinorhizobium meliloti GR4]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACAAGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT28632 WP_003527850.1 NC_019849:c1685290-1685042 [Sinorhizobium meliloti GR4]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT28632 NC_019849:c1685290-1685042 [Sinorhizobium meliloti GR4]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0