Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4712943..4713753 Replicon chromosome
Accession NZ_LR130541
Organism Klebsiella pneumoniae strain AJ218 isolate AJ218

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag EW045_RS23405 Protein ID WP_004178461.1
Coordinates 4712943..4713476 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag EW045_RS23410 Protein ID WP_002887278.1
Coordinates 4713487..4713753 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW045_RS23400 4711774..4712895 + 1122 WP_009309849.1 cupin domain-containing protein -
EW045_RS23405 4712943..4713476 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
EW045_RS23410 4713487..4713753 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
EW045_RS23415 4713856..4715289 - 1434 WP_048269807.1 Cu(+)/Ag(+) sensor histidine kinase -
EW045_RS23420 4715279..4715962 - 684 WP_048269806.1 copper response regulator transcription factor CusR -
EW045_RS23425 4716135..4717520 + 1386 WP_048269805.1 efflux transporter outer membrane subunit -
EW045_RS23430 4717538..4717882 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T286312 WP_004178461.1 NZ_LR130541:c4713476-4712943 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT286312 WP_002887278.1 NZ_LR130541:c4713753-4713487 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References