Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-Xre
Location 972104..973036 Replicon plasmid pRmeGR4d
Accession NC_019849
Organism Sinorhizobium meliloti GR4

Toxin (Protein)


Gene name - Uniprot ID Q92VJ4
Locus tag C770_RS46390 Protein ID WP_010975576.1
Coordinates 972104..972658 (-) Length 185 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID H0G7T7
Locus tag C770_RS46395 Protein ID WP_003534594.1
Coordinates 972665..973036 (-) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C770_RS46370 967636..968502 + 867 WP_010975580.1 sulfate ABC transporter permease subunit CysT -
C770_RS46375 968492..969367 + 876 WP_003537156.1 sulfate ABC transporter permease subunit CysW -
C770_RS46380 969383..970486 + 1104 WP_010975578.1 sulfate/molybdate ABC transporter ATP-binding protein -
C770_RS46385 970598..971968 + 1371 WP_015243148.1 FAD-binding oxidoreductase -
C770_RS46390 972104..972658 - 555 WP_010975576.1 RES family NAD+ phosphorylase Toxin
C770_RS46395 972665..973036 - 372 WP_003534594.1 DUF2384 domain-containing protein Antitoxin
C770_RS46400 973113..973898 - 786 WP_003534600.1 crotonase/enoyl-CoA hydratase family protein -
C770_RS46405 973895..974764 - 870 WP_015243149.1 hydroxymethylglutaryl-CoA lyase -
C770_RS46410 974761..976749 - 1989 WP_015243150.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
C770_RS46415 976766..977149 - 384 WP_010975573.1 RidA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..1701381 1701381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(23-177)

Antitoxin

(71-120)


Sequences


Toxin        


Download         Length: 185 a.a.        Molecular weight: 20459.35 Da        Isoelectric Point: 6.4901

>T28631 WP_010975576.1 NC_019849:c972658-972104 [Sinorhizobium meliloti GR4]
MRKHIPPASRPDPEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Download         Length: 555 bp

>T28631 NC_019849:c972658-972104 [Sinorhizobium meliloti GR4]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATCCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGGA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13113.26 Da        Isoelectric Point: 9.9996

>AT28631 WP_003534594.1 NC_019849:c973036-972665 [Sinorhizobium meliloti GR4]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Download         Length: 372 bp

>AT28631 NC_019849:c973036-972665 [Sinorhizobium meliloti GR4]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q92VJ4


Antitoxin

Source ID Structure
AlphaFold DB H0G7T7

References