Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7659..8302 Replicon plasmid 2
Accession NZ_LR130540
Organism Klebsiella variicola strain AJ055 isolate AJ055

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag EW050_RS27095 Protein ID WP_001044770.1
Coordinates 7886..8302 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag EW050_RS27090 Protein ID WP_001261282.1
Coordinates 7659..7889 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW050_RS27060 3172..3438 - 267 WP_024144810.1 type II toxin-antitoxin system ParD family antitoxin -
EW050_RS27065 3669..5342 - 1674 WP_000026497.1 hypothetical protein -
EW050_RS27075 5907..6716 - 810 WP_000654269.1 hypothetical protein -
EW050_RS27080 6734..7096 - 363 WP_000533745.1 hypothetical protein -
EW050_RS27085 7280..7702 - 423 WP_172601094.1 hypothetical protein -
EW050_RS27090 7659..7889 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EW050_RS27095 7886..8302 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
EW050_RS27100 8376..9938 + 1563 WP_004206609.1 AAA family ATPase -
EW050_RS27105 9923..10945 + 1023 WP_117253823.1 DNA helicase UvrD -
EW050_RS27110 11483..12397 + 915 WP_031592169.1 HNH endonuclease -
EW050_RS27115 12583..12933 - 351 WP_129671151.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..165705 165705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T286300 WP_001044770.1 NZ_LR130540:7886-8302 [Klebsiella variicola]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT286300 WP_001261282.1 NZ_LR130540:7659-7889 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References